Validation Data Gallery
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Tested Applications
| Positive WB detected in | human blood tissue, mouse serum, human plasma |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
15736-1-AP targets Hemopexin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Hemopexin fusion protein Ag8533 相同性解析による交差性が予測される生物種 |
| Full Name | hemopexin |
| Calculated molecular weight | 254 aa, 29 kDa |
| Observed molecular weight | 65-75 kDa |
| GenBank accession number | BC005395 |
| Gene Symbol | Hemopexin |
| Gene ID (NCBI) | 3263 |
| RRID | AB_2878177 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02790 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Hemopexin antibody 15736-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Br J Cancer Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins. | ||
J Cell Biochem Differential protein expression profiling by iTRAQ-2D-LC-MS/MS of rats treated with oxaliplatin. | ||
Placenta SWATH proteomics analysis of placental tissue with intrahepatic cholestasis of pregnancy | ||
Elife Myoglobin-derived iron causes wound enlargement and impaired regeneration in pressure injuries of muscle |