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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HRAS Polyclonal antibody

HRAS Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15531-1-AP

Synonyms

GTPase HRas, H RASIDX, HRAS1, EC:3.6.5.2, CTLO

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HRAS Polyclonal antibody (15531-1-AP)

    WB analysis of HeLa using 15531-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15531-1-AP (HRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using HRAS Polyclonal antibody (15531-1-AP)

    WB analysis of MCF-7 using 15531-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 15531-1-AP (HRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using HRAS Polyclonal antibody (15531-1-AP)

    WB analysis of PC-12 using 15531-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 15531-1-AP (HRAS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using HRAS Polyclonal antibody (15531-1-AP)

    WB analysis of PC-12 using 15531-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 15531-1-AP (HRAS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HRAS Polyclonal antibody (15531-1-AP)

    IP experiment of HeLa using 15531-1-AP

    IP result of anti-HRAS (IP:15531-1-AP, 4ug; Detection:15531-1-AP 1:300) with HeLa cells lysate 2600 ug.

  • Immunohistochemistry (IHC) staining of mouse intestine using HRAS Polyclonal antibody (15531-1-AP)

    IHC staining of mouse intestine using 15531-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 15531-1-AP (HRAS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using HRAS Polyclonal antibody (15531-1-AP)

    IHC staining of human stomach using 15531-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15531-1-AP (HRAS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, PC-12 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach tissue, mouse intestine
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

15531-1-AP targets HRAS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HRAS fusion protein Ag7704 相同性解析による交差性が予測される生物種
Full Name v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC006499
Gene symbol HRAS
Gene ID (NCBI) 3265
RRIDAB_2878147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HRAS antibody 15531-1-APDownload protocol
IHC protocol for HRAS antibody 15531-1-APDownload protocol
IP protocol for HRAS antibody 15531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

Sirtuin 2 knockdown inhibits cell proliferation and RAS/ERK signaling, and promotes cell apoptosis and cell cycle arrest in multiple myeloma.

Authors - Tianling Ding
View Article
humanWB

Naunyn Schmiedebergs Arch Pharmacol

The potential mechanism of Neu5Gc inducing colorectal cancer based on network pharmacology and experimental validation

Authors - Li-Chao Zhang
View Article
humanWB

Oncogene

Inhibition and degradation of NRAS with a pan-NRAS monobody

Authors - Michael Whaby
View Article