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KD/KO Validated

HRC Polyclonal antibody

Name: HRC Polyclonal antibody
Brand: Proteintech
SKU: 18142-1-AP

HRC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18142-1-AP

Synonyms

Sarcoplasmic reticulum histidine-rich calcium-binding protein

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of mouse heart using 18142-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 18142-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18142-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18142-1-AP (HRC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse heart using 18142-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 18142-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18142-1-AP (HRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 18142-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 18142-1-AP (HRC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 18142-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse heart tissue, mouse skeletal muscle tissue, HeLa cells
Positive IHC detected in	mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

18142-1-AP targets HRC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HRC fusion protein Ag12794 相同性解析による交差性が予測される生物種
Full Name	histidine rich calcium binding protein
Calculated molecular weight	699 aa, 80 kDa
Observed molecular weight	140-150 kDa
GenBank accession number	BC069795
Gene symbol	HRC
Gene ID (NCBI)	3270
RRID	AB_10598013
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for HRC antibody 18142-1-AP	Download protocol
IHC protocol for HRC antibody 18142-1-AP	Download protocol
IF protocol for HRC antibody 18142-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Elife Single-cell RNA-seq of heart reveals intercellular communication drivers of myocardial fibrosis in diabetic cardiomyopathy Authors - Wei Li View Article
mouse	WB	Chem Biol Interact Vitamin D alleviates liver fibrosis by inhibiting histidine-rich calcium binding protein (HRC). Authors - Wanyi Lu View Article
human	WB,IHC	J Adv Res Inhibition of lung cancer by vitamin D depends on downregulation of histidine-rich calcium-binding protein. Authors - Ning Liu KO Validated View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HRC Polyclonal antibody

HRC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of mouse heart using 18142-1-AP

WB analysis of mouse skeletal muscle using 18142-1-AP

WB analysis of HeLa using 18142-1-AP

IHC staining of mouse heart using 18142-1-AP

IHC staining of mouse heart using 18142-1-AP

IF Staining of HeLa using 18142-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Single-cell RNA-seq of heart reveals intercellular communication drivers of myocardial fibrosis in diabetic cardiomyopathy

Vitamin D alleviates liver fibrosis by inhibiting histidine-rich calcium binding protein (HRC).

Inhibition of lung cancer by vitamin D depends on downregulation of histidine-rich calcium-binding protein.