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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HRD1/SYVN1 Monoclonal antibody (67488-1-Ig)

    WB analysis using 67488-1-Ig (same clone as 67488-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using HRD1/SYVN1 Monoclonal antibody (67488-1-Ig)

    WB analysis of NIH/3T3 using 67488-1-Ig (same clone as 67488-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using HRD1/SYVN1 Monoclonal antibody (67488-1-Ig)

    WB analysis of 4T1 using 67488-1-Ig (same clone as 67488-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HRD1/SYVN1 Monoclonal antibody (67488-1-Ig)

    WB analysis of HeLa using 67488-1-Ig (same clone as 67488-1-PBS)

    WB result of HRD1/SYVN1 antibody (67488-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRD1/SYVN1 transfected HeLa cells. This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HRD1/SYVN1 Monoclonal antibody (67488-1-Ig)

    IHC staining of human liver cancer using 67488-1-Ig (same clone as 67488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HRD1/SYVN1 Monoclonal antibody (67488-1-Ig)

    IHC staining of human liver cancer using 67488-1-Ig (same clone as 67488-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67488-1-Ig (HRD1/SYVN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HRD1/SYVN1 Monoclonal antibody (67488-1-Ig)

    IF Staining of HeLa using 67488-1-Ig (same clone as 67488-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HRD1/SYVN1 antibody (67488-1-Ig, Clone: 2C8A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67488-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

HRD1/SYVN1 Monoclonal antibody, PBS Only

HRD1/SYVN1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67488-1-PBS
Clone No.2C8A7

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SYVN1, Synovial apoptosis inhibitor 1, KIAA1810, HRD1, EC:2.3.2.27

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67488-1-PBS targets HRD1/SYVN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HRD1/SYVN1 fusion protein Ag4456 相同性解析による交差性が予測される生物種
Full Name synovial apoptosis inhibitor 1, synoviolin
Calculated molecular weight 617 aa, 68 kDa
Observed molecular weight 68-76 kDa
GenBank accession numberBC030530
Gene Symbol HRD1
Gene ID (NCBI) 84447
RRIDAB_2882714
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86TM6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).