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  • KD/KO Validated

HRH1 Polyclonal antibody

HRH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA, IF

Conjugate

Unconjugated

Cat no : 13413-1-AP

Synonyms

H1 R, H1R, HH1R, hisH1, Histamine H1 receptor, histamine receptor H1, HRH1


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Tested Applications

Positive WB detected inhuman colon tissue, Jurkat cells
Positive IHC detected inhuman endometrial cancer tissue, human colon cancer tissue, human trachea tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13413-1-AP targets HRH1 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HRH1 fusion protein Ag4067 相同性解析による交差性が予測される生物種
Full Name histamine receptor H1
Calculated molecular weight 487 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC060802
Gene symbol HRH1
Gene ID (NCBI) 3269
RRIDAB_2092454
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HRH1 antibody 13413-1-APDownload protocol
IHC protocol for HRH1 antibody 13413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Stem Cell Reports

Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids.

Authors - Keiichiro Uehara
humanWB

Aging (Albany NY)

Combined impacts of histamine receptor H1 gene polymorphisms and an environmental carcinogen on the susceptibility to and progression of oral squamous cell carcinoma.

Authors - Yi-Fang Ding
mouseWB

Front Cell Neurosci

Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus.

Authors - Wen-Jun Su
humanWB,IHC,IF

J Virol

Kaposi's Sarcoma-Associated Herpesvirus Infection Induces the Expression of Neuroendocrine Genes in Endothelial Cells.

Authors - Mohanan Valiya Veettil
  • KO Validated
ratWB

Sci Rep

Gold nanoclusters eliminate obesity induced by antipsychotics.

Authors - Meng He
mouseIF

Am J Physiol Cell Physiol

Functional role of histamine receptors in the renal cortical collecting duct cells.

Authors - Anastasia V Sudarikova