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  • Featured Product
  • KD/KO Validated

HRPT2, CDC73 Monoclonal antibody, PBS Only

HRPT2, CDC73 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4B4H2

Cat no : 66490-1-PBS

Synonyms

C1orf28, CDC73, HPT JT, HRPT2, HRPT2; CDC73, Hyperparathyroidism 2 protein, Parafibromin

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HRPT2, CDC73 Monoclonal antibody (66490-1-Ig)

    WB analysis of HeLa using 66490-1-Ig (same clone as 66490-1-PBS)

    WB result of HRPT2, CDC73 antibody (66490-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRPT2, CDC73 transfected HeLa cells. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HRPT2, CDC73 Monoclonal antibody (66490-1-Ig)

    WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HRPT2, CDC73 Monoclonal antibody (66490-1-Ig)

    WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HRPT2, CDC73 Monoclonal antibody (66490-1-Ig)

    WB analysis using 66490-1-Ig (same clone as 66490-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using HRPT2, CDC73 Monoclonal antibody (66490-1-Ig)

    WB analysis of pig brain using 66490-1-Ig (same clone as 66490-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using HRPT2, CDC73 Monoclonal antibody (66490-1-Ig)

    WB analysis of mouse brain using 66490-1-Ig (same clone as 66490-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HRPT2, CDC73 Monoclonal antibody (66490-1-Ig)

    IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2, CDC73 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HRPT2, CDC73 Monoclonal antibody (66490-1-Ig)

    IHC staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66490-1-Ig (HRPT2; CDC73 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using HRPT2, CDC73 Monoclonal antibody (66490-1-Ig)

    IF Staining of human breast cancer using 66490-1-Ig (same clone as 66490-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using HRPT2, CDC73 antibody (66490-1-Ig, Clone: 4B4H2 ) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66490-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66490-1-PBS targets HRPT2, CDC73 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HRPT2, CDC73 fusion protein Ag2959 相同性解析による交差性が予測される生物種
Full Name cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Calculated molecular weight 531 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC014351
Gene symbol CDC73
Gene ID (NCBI) 79577
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).