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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HS3ST1 Polyclonal antibody

HS3ST1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14358-1-AP

Synonyms

3 OST 1, 3OST, 3OST1, h3 OST 1, HS3ST1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using HS3ST1 Polyclonal antibody (14358-1-AP)

    WB analysis of mouse spleen using 14358-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14358-1-AP (HS3ST1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using HS3ST1 Polyclonal antibody (14358-1-AP)

    WB analysis of mouse spleen using 14358-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14358-1-AP (HS3ST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using HS3ST1 Polyclonal antibody (14358-1-AP)

    IHC staining of human heart using 14358-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14358-1-AP (HS3ST1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using HS3ST1 Polyclonal antibody (14358-1-AP)

    IHC staining of human heart using 14358-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14358-1-AP (HS3ST1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HS3ST1 Polyclonal antibody (14358-1-AP)

    IHC staining of human kidney using 14358-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14358-1-AP (HS3ST1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HS3ST1 Polyclonal antibody (14358-1-AP)

    IHC staining of human kidney using 14358-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14358-1-AP (HS3ST1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse spleen tissue
Positive IHC detected inhuman heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14358-1-AP targets HS3ST1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HS3ST1 fusion protein Ag5719 相同性解析による交差性が予測される生物種
Full Name heparan sulfate (glucosamine) 3-O-sulfotransferase 1
Calculated molecular weight 36 kDa
GenBank accession numberBC057803
Gene symbol HS3ST1
Gene ID (NCBI) 9957
RRIDAB_10805763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HS3ST1 antibody 14358-1-APDownload protocol
IHC protocol for HS3ST1 antibody 14358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Oncol

Development and validation of a novel hypoxia-related signature for prognostic and immunogenic evaluation in head and neck squamous cell carcinoma

Authors - Su-Ran Li
View Article
humanIF

Sci Rep

Switching mechanism from AR to EGFR signaling via 3-O-sulfated heparan sulfate in castration-resistant prostate cancer

Authors - Hayato Ota
View Article