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HSD11B1 Polyclonal antibody

HSD11B1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10928-1-AP

Synonyms

7-oxosteroid reductase, 11-DH, 11-beta-hydroxysteroid dehydrogenase 1, 11-beta-HSD1, 11 DH


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Tested Applications

Positive WB detected inL02 cells, mouse liver tissue, Jurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10928-1-AP targets HSD11B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSD11B1 fusion protein Ag1361 相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (11-beta) dehydrogenase 1
Calculated molecular weight 36 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC012593
Gene symbol HSD11B1
Gene ID (NCBI) 3290
RRIDAB_2119527
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSD11B1(Corticosteroid 11-beta-dehydrogenase isozyme 1) is also named as HSD11, HSD11L,11-DH and 11-beta-HSD1.It is a microsomal enzyme, which acts mainly as a NADP(H)- dependent reductase converting inactive cortisone (E) to active cortisol (F), thus regulating intracellular F access to glucocorticoid receptor. This enzyme is widely expressed not only in liver and adipose tissue, but also in adrenal, ovary and decidua.Western blot analysis in human liver, using a specific11-beta-HSD1 antibody, showed two bands at approximately 34 and 68 kDa confirming the dimeric nature of 11-beta-HSD1 in liver. In omental adipose tissue, an additional band of 50 kDa was strongly expressed(PMID:16914598).

Protocols

Product Specific Protocols
WB protocol for HSD11B1 antibody 10928-1-APDownload protocol
IF protocol for HSD11B1 antibody 10928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Brain Behav Immun

HMGB1-mediated differential response on hippocampal neurotransmitter disorder and neuroinflammation in adolescent male and female mice following cold exposure.

Authors - Bin Xu
humanWB

J Assist Reprod Genet

Effect of luteinizing hormone concentration on transcriptome and subcellular organelle phenotype of ovarian granulosa cells.

Authors - Yu-Ting Wan
mouseIF,WB,IHC

Int J Biol Sci

11β-Hydroxysteroid Dehydrogenase Type 1 Facilitates Osteoporosis by Turning on Osteoclastogenesis through Hippo Signaling

Authors - Hanwen Li
humanWB

Gynecol Endocrinol

N6-methyladenosine demethylase FTO related to hyperandrogenism in PCOS via AKT pathway

Authors - Yuan-Xue Jing