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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSD17B1 Polyclonal antibody

HSD17B1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27830-1-AP

Synonyms

17 beta HSD 1, 20 alpha HSD, E17KSR, E2DH, EDH17B1, EDH17B2, EDHB17, HSD17, HSD17B1, SDR28C1

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using HSD17B1 Polyclonal antibody (27830-1-AP)

    WB analysis of human placenta using 27830-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27830-1-AP (HSD17B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using HSD17B1 Polyclonal antibody (27830-1-AP)

    WB analysis of human placenta using 27830-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27830-1-AP (HSD17B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using HSD17B1 Polyclonal antibody (27830-1-AP)

    WB analysis of human placenta using 27830-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27830-1-AP (HSD17B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using HSD17B1 Polyclonal antibody (27830-1-AP)

    IHC staining of human placenta using 27830-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27830-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HSD17B1 Polyclonal antibody (27830-1-AP)

    IHC staining of human placenta using 27830-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27830-1-AP (HSD17B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HSD17B1 Polyclonal antibody (27830-1-AP)

    IHC staining of human placenta using 27830-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27830-1-AP (HSD17B1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HSD17B1 Polyclonal antibody (27830-1-AP)

    IHC staining of human placenta using 27830-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27830-1-AP (HSD17B1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27830-1-AP targets HSD17B1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSD17B1 fusion protein Ag27213 相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (17-beta) dehydrogenase 1
Calculated molecular weight 328 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC104752
Gene symbol HSD17B1
Gene ID (NCBI) 3292
RRIDAB_2880985
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HSD17B1 antibody 27830-1-APDownload protocol
IHC protocol for HSD17B1 antibody 27830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols