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ERAB Polyclonal antibody

Name: ERAB Polyclonal antibody
Brand: Proteintech
SKU: 10648-1-AP

ERAB Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10648-1-AP

Synonyms

HSD17B10, 3-hydroxyacyl-CoA dehydrogenase type II, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, 3alpha(or 20beta)-hydroxysteroid dehydrogenase, 3-alpha-(17-beta)-hydroxysteroid dehydrogenase

Validation Data Gallery

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AllWBIPIHC

WB analysis of HeLa using 10648-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10648-1-AP
WB result of ERAB antibody (10648-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERAB transfected HEK-293 cells.
WB analysis of HeLa using 10648-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10648-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10648-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A2780 using 10648-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10648-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10648-1-AP (ERAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10648-1-AP
IP result of anti-ERAB (IP:10648-1-AP, 4ug; Detection:10648-1-AP 1:500) with HeLa cells lysate 1200ug.
IHC staining of human breast cancer using 10648-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10648-1-AP (ERAB antibody) at dilution of 1:100 (under 10x lens).
IHC staining of mouse brain using 10648-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10648-1-AP (ERAB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 10648-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue, HEK-293 cells, A2780 cells, mouse liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IP	See 1 publications below

Product Information

10648-1-AP targets ERAB in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ERAB fusion protein Ag1020 相同性解析による交差性が予測される生物種
Full Name	hydroxysteroid (17-beta) dehydrogenase 10
Calculated molecular weight	26 kDa
Observed molecular weight	27 kDa
GenBank accession number	BC008708
Gene symbol	ERAB
Gene ID (NCBI)	3028
RRID	AB_2264270
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HSD17B10 (3-hydroxyacyl-CoA dehydrogenase type-2) is a multifunctional mitochondrial enzyme that acts on a wide spectrum of substrates, including neuroactive steroids, alcohols, leucine, and fatty acids, with a preference for short-chain methyl-branched acyl-CoAs(PMID:15860413).It has 2 isoforms produced by alternative splicing.Defects in HSD17B10 are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency) and mental retardation syndromic X-linked type 10 (MRXS10).

Protocols

Product Specific Protocols
WB protocol for ERAB antibody 10648-1-AP	Download protocol
IHC protocol for ERAB antibody 10648-1-AP	Download protocol
IP protocol for ERAB antibody 10648-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Rep Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly. Authors - Oliver Rackham View Article
human	WB	Hum Mol Genet Leber's hereditary optic neuropathy (LHON)-associated ND6 14 484 T > C mutation caused pleiotropic effects on the complex I, RNA homeostasis, apoptosis and mitophagy. Authors - Min Liang View Article
mouse	WB	Endocrinology Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice Authors - Abraham Behrmann View Article
rat	WB	J Oleo Sci Effects of Dietary Fat Restriction on Endurance Training-induced Metabolic Adaptations in Rat Skeletal Muscle. Authors - Takuya Karasawa View Article
mouse	WB	Circulation Stimulates Short-Chain Dehydrogenase/Reductase Proteins to Alleviate Heart Failure Independent of Mitochondrial Protein Deacetylation Authors - Matthew A Walker View Article
rat	WB	Gen Comp Endocrinol Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats Authors - Dong Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ERAB Polyclonal antibody

ERAB Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 10648-1-AP

WB analysis of HEK-293 using 10648-1-AP

WB analysis of HeLa using 10648-1-AP

WB analysis of HEK-293 using 10648-1-AP

WB analysis of mouse liver using 10648-1-AP

WB analysis of A2780 using 10648-1-AP

WB analysis of human brain using 10648-1-AP

IP experiment of HeLa using 10648-1-AP

IHC staining of human breast cancer using 10648-1-AP

IHC staining of mouse brain using 10648-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.

Leber's hereditary optic neuropathy (LHON)-associated ND6 14 484 T > C mutation caused pleiotropic effects on the complex I, RNA homeostasis, apoptosis and mitophagy.

Wnt16 Promotes Vascular Smooth Muscle Contractile Phenotype and Function via Taz (Wwtr1) Activation in Male LDLR-/- Mice

Effects of Dietary Fat Restriction on Endurance Training-induced Metabolic Adaptations in Rat Skeletal Muscle.

Stimulates Short-Chain Dehydrogenase/Reductase Proteins to Alleviate Heart Failure Independent of Mitochondrial Protein Deacetylation

Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats