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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSD17B11 Polyclonal antibody

HSD17B11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16303-1-AP

Synonyms

17-beta-HSD XI, 17-beta-HSD 11, 17 BETA HSDXI, 17 BETA HSD11, 17 beta HSD XI

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using HSD17B11 Polyclonal antibody (16303-1-AP)

    WB analysis of L02 using 16303-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using HSD17B11 Polyclonal antibody (16303-1-AP)

    WB analysis of mouse liver using 16303-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using HSD17B11 Polyclonal antibody (16303-1-AP)

    WB analysis of mouse lung using 16303-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using HSD17B11 Polyclonal antibody (16303-1-AP)

    WB analysis of mouse liver using 16303-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using HSD17B11 Polyclonal antibody (16303-1-AP)

    WB analysis of mouse heart using 16303-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using HSD17B11 Polyclonal antibody (16303-1-AP)

    WB analysis of mouse lung using 16303-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16303-1-AP (HSD17B11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using HSD17B11 Polyclonal antibody (16303-1-AP)

    IHC staining of human ovary using 16303-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16303-1-AP (HSD17B11 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using HSD17B11 Polyclonal antibody (16303-1-AP)

    IHC staining of human ovary using 16303-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16303-1-AP (HSD17B11 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using HSD17B11 Polyclonal antibody (16303-1-AP)

    IHC staining of human ovary cancer using 16303-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16303-1-AP (HSD17B11 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inL02 cells, mouse heart tissue, mouse liver tissue, mouse lung tissue
Positive IHC detected inhuman ovary cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16303-1-AP targets HSD17B11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSD17B11 fusion protein Ag9278 相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (17-beta) dehydrogenase 11
Calculated molecular weight 300 aa, 33 kDa
Observed molecular weight 28-33 kDa
GenBank accession numberBC021673
Gene symbol HSD17B11
Gene ID (NCBI) 51170
RRIDAB_2878241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HSD17B11 antibody 16303-1-APDownload protocol
IHC protocol for HSD17B11 antibody 16303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Biosci

m6A demethylase FTO promotes tumor progression via regulation of lipid metabolism in esophageal cancer.

Authors - Xiaoran Duan
View Article