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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSD17B11 Polyclonal antibody (17301-1-AP)

    WB analysis using 17301-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17301-1-AP (HSD17B11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using HSD17B11 Polyclonal antibody (17301-1-AP)

    IHC staining of human kidney using 17301-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HSD17B11 Polyclonal antibody (17301-1-AP)

    IHC staining of human kidney using 17301-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using HSD17B11 Polyclonal antibody (17301-1-AP)

    IHC staining of human brain using 17301-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using HSD17B11 Polyclonal antibody (17301-1-AP)

    IHC staining of human brain using 17301-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using HSD17B11 Polyclonal antibody (17301-1-AP)

    IHC staining of human ovary using 17301-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using HSD17B11 Polyclonal antibody (17301-1-AP)

    IHC staining of human ovary using 17301-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17301-1-AP (HSD17B11 antibody) at dilution of 1:100 (under 40x lens).

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HSD17B11 Polyclonal antibody

HSD17B11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17301-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

17 beta HSD 11, 17 beta HSD XI, 17 BETA HSD11, 17 BETA HSDXI, 17betaHSD11, 17betaHSDXI, 17bHSD11, DHRS8, HSD17B11, PAN1B, RETSDR2, SDR16C2, UNQ207/PRO233

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse pancreas tissue, rat pancreas，
Positive IHC detected inhuman kidney tissue, human brain tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

17301-1-AP targets HSD17B11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSD17B11 fusion protein Ag11182 相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (17-beta) dehydrogenase 11
Calculated molecular weight 300 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC016367
Gene Symbol HSD17B11
Gene ID (NCBI) 51170
RRIDAB_2119810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBQ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HSD17B11 antibody 17301-1-APDownload protocol
IHC protocol for HSD17B11 antibody 17301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Syst Biol Reprod Med

Identification of ferroptotic genes and phenotypes in idiopathic nonobstructive azoospermia

Authors - Chen Liao
View Article