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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSD17B12 Polyclonal antibody

HSD17B12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27461-1-AP

Synonyms

EC:1.1.1.330, 3-ketoacyl-CoA reductase, 3 ketoacyl CoA reductase, 17-beta-hydroxysteroid dehydrogenase 12, 17-beta-HSD 12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSD17B12 Polyclonal antibody (27461-1-AP)

    WB analysis using 27461-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27461-1-AP (HSD17B12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inCaco-2 cells, HCT 116 cells, HEK-293 cells, HT-29 cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27461-1-AP targets HSD17B12 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSD17B12 fusion protein Ag26540 相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (17-beta) dehydrogenase 12
Calculated molecular weight 312 aa, 34 kDa
Observed molecular weight30 kDa
GenBank accession numberBC012043
Gene symbol HSD17B12
Gene ID (NCBI) 51144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hydroxysteroid 17-beta dehydrogenase 12 (HSD17B12, also known as KAR and SDR12C1) is a key enzyme of steroid metabolism pathway19 and is the human homolog of the yeast 3-ketoacyl-CoA reductase that catalyzes the second reaction in each VLCFA elongation cycle (PMID: 16166196; 12482854). It also acts as a drug-metabolizing enzyme (PMID: 36724833). HSD17B12 deficiency in embryonic stem cells reduced the production of arachidonic acid, a precursor of prostaglandins (PMID: 20130115).

Protocols

Product Specific Protocols
WB protocol for HSD17B12 antibody 27461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols