Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, MCF-7 cells, human placenta tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 17 publications below |
| IHC | See 21 publications below |
Product Information
10978-1-AP targets HSD17B2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HSD17B2 fusion protein Ag1411 相同性解析による交差性が予測される生物種 |
| Full Name | hydroxysteroid (17-beta) dehydrogenase 2 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC009581 |
| Gene Symbol | HSD17B2 |
| Gene ID (NCBI) | 3294 |
| RRID | AB_2279863 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P37059 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSD17B2(Estradiol 17-beta-dehydrogenase 2) is also named as 17-beta-HSD 2,20-alpha-HSD,EDH17B2 and belongs to the short-chain dehydrogenases/reductases (SDR) family.Based on the pivotal role of HSD17B2 gene product in the estrogen metabolism pathway, it may be considered as a putative candidate gene that could possibly explain a fraction of the remaining familial breast cancer risk and it is unlikely to play a role as a high penetrance gene in breast cancer predisposition(PMID:18372405).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSD17B2 antibody 10978-1-AP | Download protocol |
| IHC protocol for HSD17B2 antibody 10978-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Part Fibre Toxicol Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway. | ||
J Clin Endocrinol Metab MUC1 as a discriminator between endometrium from fertile and infertile patients with PCOS and endometriosis. | ||
Br J Cancer In breast cancer subtypes steroid sulfatase (STS) is associated with less aggressive tumour characteristics. | ||
Fertil Steril Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome. | ||
Ecotoxicol Environ Saf Cypermethrin triggers YY1-mediated testosterone biosynthesis suppression. |