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HSD17B4 Polyclonal antibody

Name: HSD17B4 Polyclonal antibody
Brand: Proteintech
SKU: 15116-1-AP

HSD17B4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15116-1-AP

Synonyms

D-bifunctional protein, D bifunctional protein, 17-beta-hydroxysteroid dehydrogenase 4, 17-beta-HSD 4, 17 beta HSD 4

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 15116-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15116-1-AP
WB result of HSD17B4 antibody (15116-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B4 transfected HepG2 cells.
WB analysis of mouse brain using 15116-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 15116-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15116-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 15116-1-AP
IP result of anti-HSD17B4 (IP:15116-1-AP, 4ug; Detection:15116-1-AP 1:500) with mouse brain tissue lysate 4000ug.
IHC staining of human prostate cancer using 15116-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 15116-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 15116-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse liver using 15116-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse heart using 15116-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse heart using 15116-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human prostate cancer using 15116-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15116-1-AP (HSD17B4 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human prostate cancer using 15116-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15116-1-AP (HSD17B4 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HeLa using 15116-1-AP
Immunofluorescent analysis of Hela cells, using HSD17B4 antibody 15116-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 15116-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, mouse brain tissue, mouse heart tissue, HepG2 cells, rat liver tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human prostate cancer tissue, mouse liver tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 15 publications below

Product Information

15116-1-AP targets HSD17B4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HSD17B4 fusion protein Ag7165 相同性解析による交差性が予測される生物種
Full Name	hydroxysteroid (17-beta) dehydrogenase 4
Calculated molecular weight	80 kDa
Observed molecular weight	80 kDa, 45 kDa
GenBank accession number	BC003098
Gene symbol	HSD17B4
Gene ID (NCBI)	3295
RRID	AB_2119959
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HSD17B4 (17-beta-hydroxysteroid dehydrogenase 4) is also named as Peroxisomal multifunctional enzyme type 2, D-bifuntional protein or multifunctional protein 2. It codes for a 80 kDa enzyme containing three distinct functional domains and is localized in peroxisomes. It is a bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids and catalyzing the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-brancked-chian fatty acids. After peroxisomal import, the full-length protein is proteolytically cleaved to yield a 35-kDa dehydrogenase subunit and a 45-kDa hydratase subunit containing the hydratase and SCP domains (PMID: 28868548, 24602372).

Protocols

Product Specific Protocols
WB protocol for HSD17B4 antibody 15116-1-AP	Download protocol
IHC protocol for HSD17B4 antibody 15116-1-AP	Download protocol
IF protocol for HSD17B4 antibody 15116-1-AP	Download protocol
IP protocol for HSD17B4 antibody 15116-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Commun Biol Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism Authors - Michael Landowski View Article
mouse	WB	Neurobiol Dis Peroxisomal multifunctional protein-2 deficiency causes neuroinflammation and degeneration of Purkinje cells independent of very long chain fatty acid accumulation. Authors - Verheijden Simon S View Article
	WB	Sci Rep Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells. Authors - Mitsuki Nozaki View Article
mouse	WB	Cells Cell Type-Selective Loss of Peroxisomal β-Oxidation Impairs Bipolar Cell but Not Photoreceptor Survival in the Retina. Authors - Daniëlle Swinkels View Article
human	WB	Mol Cells The Peroxisomal Localization of Hsd17b4 Is Regulated by Its Interaction with Phosphatidylserine. Authors - Sang-Ah Lee View Article
human	WB	Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. Authors - Olga Gourdomichali View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSD17B4 Polyclonal antibody

HSD17B4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 15116-1-AP

WB analysis of HepG2 using 15116-1-AP

WB analysis of mouse brain using 15116-1-AP

WB analysis of mouse heart using 15116-1-AP

WB analysis of mouse liver using 15116-1-AP

IP experiment of mouse brain using 15116-1-AP

IHC staining of human prostate cancer using 15116-1-AP

IHC staining of human prostate cancer using 15116-1-AP

IHC staining of mouse liver using 15116-1-AP

IHC staining of mouse liver using 15116-1-AP

IHC staining of mouse heart using 15116-1-AP

IHC staining of mouse heart using 15116-1-AP

IHC staining of human prostate cancer using 15116-1-AP

IHC staining of human prostate cancer using 15116-1-AP

IF Staining of HeLa using 15116-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism

Peroxisomal multifunctional protein-2 deficiency causes neuroinflammation and degeneration of Purkinje cells independent of very long chain fatty acid accumulation.

Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells.

Cell Type-Selective Loss of Peroxisomal β-Oxidation Impairs Bipolar Cell but Not Photoreceptor Survival in the Retina.

The Peroxisomal Localization of Hsd17b4 Is Regulated by Its Interaction with Phosphatidylserine.

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.