HSD17B6 Polyclonal antibody

Name: HSD17B6 Polyclonal antibody
Brand: Proteintech
SKU: 11855-1-AP

HSD17B6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11855-1-AP

Synonyms

17 beta HSD 6, 17 beta HSD6, 3 alpha >beta HSE, HSD17B6, HSE, RODH, SDR9C6

Validation Data Gallery

Filter:

AllWBIHC

WB analysis using 11855-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11855-1-AP (HSD17B6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11855-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 11855-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11855-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11855-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11855-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11855-1-AP (HSD17B6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate hyperplasia using 11855-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate hyperplasia using 11855-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 11855-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 11855-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11855-1-AP (HSD17B6 Antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 11855-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, human liver tissue, rat liver tissue
Positive IHC detected in	human lung cancer tissue, human prostate hyperplasia tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below

Product Information

11855-1-AP targets HSD17B6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HSD17B6 fusion protein Ag2427 相同性解析による交差性が予測される生物種
Full Name	hydroxysteroid (17-beta) dehydrogenase 6 homolog (mouse)
Calculated molecular weight	317 aa, 36 kDa
Observed molecular weight	33-35 kDa
GenBank accession number	BC020710
Gene symbol	HSD17B6
Gene ID (NCBI)	8630
RRID	AB_2295487
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for HSD17B6 antibody 11855-1-AP	Download protocol
IHC protocol for HSD17B6 antibody 11855-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Breast Cancer Res Treat The presence and impact of estrogen metabolism on the biology of triple-negative breast cancer. Authors - Keely May McNamara View Article
human	WB	Cancer Cell Int Downexpression of HSD17B6 correlates with clinical prognosis and tumor immune infiltrates in hepatocellular carcinoma. Authors - Lei Lv View Article
human	WB	Cell Death Discov HSD17B6 downregulation predicts poor prognosis and drives tumor progression via activating Akt signaling pathway in lung adenocarcinoma Authors - Tian Tian View Article
mouse	WB	Toxicol Lett Metabolomics reveals dysregulated all-trans retinoic acid and polyunsaturated fatty acid metabolism contribute to PXR-induced hepatic steatosis in mice Authors - Liang Yan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSD17B6 Polyclonal antibody

HSD17B6 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 11855-1-AP

WB analysis of human liver using 11855-1-AP

IHC staining of human lung cancer using 11855-1-AP

IHC staining of human lung cancer using 11855-1-AP

IHC staining of human lung cancer using 11855-1-AP

IHC staining of human lung cancer using 11855-1-AP

IHC staining of human prostate hyperplasia using 11855-1-AP

IHC staining of human prostate hyperplasia using 11855-1-AP

IHC staining of human liver using 11855-1-AP

IHC staining of human liver using 11855-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The presence and impact of estrogen metabolism on the biology of triple-negative breast cancer.

Downexpression of HSD17B6 correlates with clinical prognosis and tumor immune infiltrates in hepatocellular carcinoma.

HSD17B6 downregulation predicts poor prognosis and drives tumor progression via activating Akt signaling pathway in lung adenocarcinoma

Metabolomics reveals dysregulated all-trans retinoic acid and polyunsaturated fatty acid metabolism contribute to PXR-induced hepatic steatosis in mice