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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using HSD17B7 Polyclonal antibody (16925-1-AP)

    WB analysis of HEK-293 using 16925-1-AP

    WB result of HSD17B7 antibody (16925-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B7 transfected HEK-293 cells.

  • Western Blot (WB) analysis of human liver tissue using HSD17B7 Polyclonal antibody (16925-1-AP)

    WB analysis of human liver using 16925-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16925-1-AP (HSD17B7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using HSD17B7 Polyclonal antibody (16925-1-AP)

    WB analysis of L02 using 16925-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16925-1-AP (HSD17B7 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using HSD17B7 Polyclonal antibody (16925-1-AP)

    WB analysis of L02 using 16925-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16925-1-AP (HSD17B7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of L02 cells using HSD17B7 Polyclonal antibody (16925-1-AP)

    IP experiment of L02 using 16925-1-AP

    IP result of anti-HSD17B7 (IP:16925-1-AP, 4ug; Detection:16925-1-AP 1:500) with L02 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using HSD17B7 Polyclonal antibody (16925-1-AP)

    IHC staining of human liver using 16925-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16925-1-AP (HSD17B7 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using HSD17B7 Polyclonal antibody (16925-1-AP)

    IHC staining of human liver using 16925-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16925-1-AP (HSD17B7 antibody) at dilution of 1:50 (under 40x lens).

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  • Featured Product
  • KD/KO Validated

HSD17B7 Polyclonal antibody

HSD17B7 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 16925-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Synonyms

Hsd17b_2, HSD17B7, HSD17B7P2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 150 ug/ml
20 μL, 150 ug/ml
150 μL, 150 ug/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman liver tissue, L02 cells, HEK-293 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16925-1-AP targets HSD17B7 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSD17B7 fusion protein Ag10315 相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (17-beta) dehydrogenase 7 pseudogene 2
Calculated molecular weight 26 kDa, 37 kDa
Observed molecular weight 32-37 kDa
GenBank accession numberBC071987
Gene Symbol HSD17B7
Gene ID (NCBI) 158160
RRIDAB_2119975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HSD17B7 antibody 16925-1-APDownload protocol
IHC protocol for HSD17B7 antibody 16925-1-APDownload protocol
IP protocol for HSD17B7 antibody 16925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols