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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSD17B8 Polyclonal antibody

HSD17B8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16752-1-AP

Synonyms

17 beta HSD 8, D6S2245E, FABG, FABGL, H2 KE6, HKE6, HSD17B8, Ke 6, KE6, Protein Ke6, RING2, SDR30C1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSD17B8 Polyclonal antibody (16752-1-AP)

    WB analysis using 16752-1-AP

    Hela cells lysates were subjected to SDS PAGE followed by western blot with 16752-1-AP (HSD17B8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    WB analysis of human testis using 16752-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16752-1-AP (HSD17B8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    IHC staining of human liver cancer using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    IHC staining of human liver cancer using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    IHC staining of mouse kidney using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    IHC staining of mouse kidney using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    IHC staining of human liver cancer using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSD17B8 Polyclonal antibody (16752-1-AP)

    IHC staining of human liver cancer using 16752-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16752-1-AP (HSD17B8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse small intestine tissue, human testis tissue
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16752-1-AP targets HSD17B8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSD17B8 fusion protein Ag10226 相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (17-beta) dehydrogenase 8
Calculated molecular weight 261 aa, 27 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC008185
Gene symbol HSD17B8
Gene ID (NCBI) 7923
RRIDAB_2248540
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HSD17B8 antibody 16752-1-APDownload protocol
IHC protocol for HSD17B8 antibody 16752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Cell facilitation promotes growth and survival under drug pressure in breast cancer

Authors - Rena Emond
View Article