HSD3B2 Monoclonal antibody

HSD3B2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E7B4

Cat no : 67572-1-Ig

Synonyms

3-beta-HSD II, 3-beta-HSD adrenal and gonadal type, 3b HSD, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2


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Tested Applications

Positive WB detected inhuman placenta tissue, pig adrenal gland tissue, human adrenal gland tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67572-1-Ig targets HSD3B2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSD3B2 fusion protein Ag4291 相同性解析による交差性が予測される生物種
Full Name hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC038419
Gene symbol HSD3B2
Gene ID (NCBI) 3284
RRIDAB_2882785
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSD3B2(3-beta-hydroxy-Delta(5)-steroid dehydrogenase) belongs to the 3-beta-HSD family and catalyzes the oxidation and isomerization of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, thus leading to the formation of all classes of steroid hormones.HSD3B2 is expressed almost exclusively in adrenals and gonads, whereas HSD3B1 is expressed predominantly in placenta and skin(PMID:1682237). Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2).

Protocols

Product Specific Protocols
WB protocol for HSD3B2 antibody 67572-1-IgDownload protocol
IF protocol for HSD3B2 antibody 67572-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Part Fibre Toxicol

Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.

Authors - Haibo Jin
humanWB

Ecotoxicol Environ Saf

Biochemical mechanisms of tributyltin chloride-induced cell toxicity in Sertoli cells

Authors - Pengchen Chen
humanWB

Birth Defects Res

Bisphenol A decreases progesterone synthesis in human ovarian granulosa cells.

Authors - Junfeng Qi
humanWB

Gynecol Endocrinol

N6-methyladenosine demethylase FTO related to hyperandrogenism in PCOS via AKT pathway

Authors - Yuan-Xue Jing
humanIHC

Pathol Res Pract

Disorganized adrenocortical zonational structure in COVID-19 patients: Implications of critical illness duration

Authors - Tian-Zi Wen
humanIHC,IF

Metabolism

Increased adrenal steroidogenesis and suppressed corticosteroid responsiveness in critical COVID-19

Authors - Tian-Zi Wen