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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSDL2 Monoclonal antibody (68414-1-Ig)

    WB analysis using 68414-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68414-1-Ig (HSDL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSDL2 Monoclonal antibody (68414-1-Ig)

    IF Staining of HeLa using 68414-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSDL2 antibody (68414-1-Ig, Clone: 3D11G1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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HSDL2 Monoclonal antibody

HSDL2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68414-1-Ig
Clone No.3D11G1

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

C9orf99, FLJ25855, HSDL2, SDR13C1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, pig heart tissue, rabbit heart tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68414-1-Ig targets HSDL2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, rabbit samples.

Tested Reactivity Human, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HSDL2 fusion protein Ag8314 相同性解析による交差性が予測される生物種
Full Name hydroxysteroid dehydrogenase like 2
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC004331
Gene Symbol HSDL2
Gene ID (NCBI) 84263
RRIDAB_3085130
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6YN16
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human hydroxysteroid dehydrogenase-like 2 (HSDL2) is a characterized SDR gene that not only catalyses the oxidation and reduction of multiple substrates but also regulates different metabolic and signalling pathways. Accumulating evidences suggest that HSDL2 play an important role in cancer progression. HSDL2 has promoting effects on tumor progression in papillary thyroid cancer, bladder cancer, ovarian cancer, and glioma, but has suppressing effects in cholangiocarcinoma . ( PMID: 31372054, PMID: 32211805)

Protocols

Product Specific Protocols
WB protocol for HSDL2 antibody 68414-1-IgDownload protocol
IF protocol for HSDL2 antibody 68414-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols