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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using HSF1 Polyclonal antibody (16107-1-AP)

    WB analysis of K-562 using 16107-1-AP (same clone as 16107-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HSF1 Polyclonal antibody (16107-1-AP)

    WB analysis of HepG2 using 16107-1-AP (same clone as 16107-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using HSF1 Polyclonal antibody (16107-1-AP)

    WB analysis of MCF-7 using 16107-1-AP (same clone as 16107-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using HSF1 Polyclonal antibody (16107-1-AP)

    WB analysis of mouse testis using 16107-1-AP (same clone as 16107-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using HSF1 Polyclonal antibody (16107-1-AP)

    WB analysis of mouse spleen using 16107-1-AP (same clone as 16107-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using HSF1 Polyclonal antibody (16107-1-AP)

    WB analysis of mouse kidney using 16107-1-AP (same clone as 16107-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSF1 Polyclonal antibody (16107-1-AP)

    IHC staining of human lung cancer using 16107-1-AP (same clone as 16107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using HSF1 Polyclonal antibody (16107-1-AP)

    IHC staining of human cervical cancer using 16107-1-AP (same clone as 16107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using HSF1 Polyclonal antibody (16107-1-AP)

    IHC staining of human cervical cancer using 16107-1-AP (same clone as 16107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HSF1 Polyclonal antibody (16107-1-AP)

    IHC staining of human lung cancer using 16107-1-AP (same clone as 16107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

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HSF1 Polyclonal antibody, PBS Only

HSF1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16107-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16107-1-PBS targets HSF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSF1 fusion protein Ag9023 相同性解析による交差性が予測される生物種
Full Name heat shock transcription factor 1
Calculated molecular weight 529 aa, 57 kDa
Observed molecular weight 68-80 kDa
GenBank accession numberBC014638
Gene Symbol HSF1
Gene ID (NCBI) 3297
RRIDAB_11182610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00613
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).