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HSF1 Polyclonal antibody

Name: HSF1 Polyclonal antibody
Brand: Proteintech
SKU: 51034-1-AP

HSF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 51034-1-AP

Synonyms

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 51034-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 51034-1-AP
WB result of HSF1 antibody (51034-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSF1 transfected HeLa cells.
WB analysis using 51034-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 51034-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 51034-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 51034-1-AP
IP result of anti-HSF1 (IP:51034-1-AP, 4ug; Detection:51034-1-AP 1:8000) with Jurkat cells lysate 1560 ug.
IHC staining of human lung cancer using 51034-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51034-1-AP (HSF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 51034-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51034-1-AP (HSF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 51034-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51034-1-AP (HSF1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 51034-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 51034-1-AP (HSF1 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A431 using 51034-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using HSF1 antibody (51034-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 51034-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, A431 cells, HepG2 cells, K-562 cells, NIH/3T3 cells, HEK-293T cells, C6 cells, mouse ovary tissue, mouse testis tissue
Positive IP detected in	Jurkat cells
Positive IHC detected in	human lung cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 13 publications below
IHC	See 2 publications below
IF	See 4 publications below
IP	See 1 publications below

Product Information

51034-1-AP targets HSF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HSF1 fusion protein Ag0739 相同性解析による交差性が予測される生物種
Full Name	heat shock transcription factor 1
Calculated molecular weight	529 aa, 57 kDa
Observed molecular weight	68-80 kDa
GenBank accession number	BC014638
Gene symbol	HSF1
Gene ID (NCBI)	3297
RRID	AB_2120269
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).

Protocols

Product Specific Protocols
WB protocol for HSF1 antibody 51034-1-AP	Download protocol
IHC protocol for HSF1 antibody 51034-1-AP	Download protocol
IF protocol for HSF1 antibody 51034-1-AP	Download protocol
IP protocol for HSF1 antibody 51034-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Science Z-DNA binding protein 1 promotes heatstroke-induced cell death. Authors - Fangfang Yuan View Article
mouse	WB	Nat Commun Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy Authors - Donghai Cui View Article
rat	WB	Dev Cell Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination Authors - Li Li View Article
mouse	WB	Food Funct Protective effects of L-theanine and dihydromyricetin on reproductive function in male mice under heat stress Authors - Difei Yang View Article
rat		Int J Pharm Effective myocardial infarction treatment by targeted accumulation of Sulforaphane using porous magnetic silica nanoparticles Authors - Jian Zhang View Article
human	WB	Am J Physiol Cell Physiol Cancer associated fibroblasts suppressed ferroptosis in glioblastoma via upregulating lncRNA DLEU1 Authors - Jie Zhao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSF1 Polyclonal antibody

HSF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 51034-1-AP

WB analysis of HeLa using 51034-1-AP

WB analysis using 51034-1-AP

WB analysis using 51034-1-AP

WB analysis of A431 using 51034-1-AP

IP experiment of Jurkat using 51034-1-AP

IHC staining of human lung cancer using 51034-1-AP

IHC staining of human lung cancer using 51034-1-AP

IHC staining of human testis using 51034-1-AP

IHC staining of human testis using 51034-1-AP

IF Staining of A431 using 51034-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Z-DNA binding protein 1 promotes heatstroke-induced cell death.

Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy

Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination

Protective effects of L-theanine and dihydromyricetin on reproductive function in male mice under heat stress

Effective myocardial infarction treatment by targeted accumulation of Sulforaphane using porous magnetic silica nanoparticles

Cancer associated fibroblasts suppressed ferroptosis in glioblastoma via upregulating lncRNA DLEU1