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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSF1 Monoclonal antibody (67189-1-Ig)

    WB analysis using 67189-1-Ig (same clone as 67189-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67189-1-Ig (HSF1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HSF1 Monoclonal antibody (67189-1-Ig)

    WB analysis of HeLa using 67189-1-Ig (same clone as 67189-1-PBS)

    WB result of HSF1 antibody (67189-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSF1 transfected HeLa cells. This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HSF1 Monoclonal antibody (67189-1-Ig)

    IHC staining of human breast cancer using 67189-1-Ig (same clone as 67189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HSF1 Monoclonal antibody (67189-1-Ig)

    IHC staining of human breast cancer using 67189-1-Ig (same clone as 67189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HSF1 Monoclonal antibody (67189-1-Ig)

    IHC staining of human ovary tumor using 67189-1-Ig (same clone as 67189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HSF1 Monoclonal antibody (67189-1-Ig)

    IHC staining of human ovary tumor using 67189-1-Ig (same clone as 67189-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67189-1-Ig (HSF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67189-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

HSF1 Monoclonal antibody, PBS Only

HSF1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67189-1-PBS
Clone No.1F10A5

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Heat shock factor protein 1, HSF 1, HSF1, HSTF 1, HSTF1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67189-1-PBS targets HSF1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSF1 fusion protein Ag29061 相同性解析による交差性が予測される生物種
Full Name heat shock transcription factor 1
Calculated molecular weight 529 aa, 57 kDa
Observed molecular weight 68-80 kDa
GenBank accession numberBC014638
Gene Symbol HSF1
Gene ID (NCBI) 3297
RRIDAB_2882484
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ00613
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).