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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSF2 Polyclonal antibody (10706-1-AP)

    WB analysis using 10706-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10706-1-AP (HSF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HSF2 Polyclonal antibody (10706-1-AP)

    WB analysis of Jurkat using 10706-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10706-1-AP (HSF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSF2 Polyclonal antibody (10706-1-AP)

    IHC staining of mouse testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSF2 Polyclonal antibody (10706-1-AP)

    IHC staining of mouse testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using HSF2 Polyclonal antibody (10706-1-AP)

    IHC staining of rat testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using HSF2 Polyclonal antibody (10706-1-AP)

    IHC staining of rat testis using 10706-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10706-1-AP (HSF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

HSF2 Polyclonal antibody

HSF2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10706-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, Mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Heat shock factor protein 2, HSF 2, HSF2, HSTF 2, HSTF2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 600 ug/ml
20 μL, 600 ug/ml
150 μL, 600 ug/ml

¥67,000/ 150 μL

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, A549 cells
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10706-1-AP targets HSF2 in WB, IHC, ELISA applications and shows reactivity with human, Mouse, rat samples.

Tested Reactivity human, Mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSF2 fusion protein Ag1067 相同性解析による交差性が予測される生物種
Full Name heat shock transcription factor 2
Calculated molecular weight 79 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC005329
Gene Symbol HSF2
Gene ID (NCBI) 3298
RRIDAB_2120443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ03933
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HSF2 antibody 10706-1-APDownload protocol
IHC protocol for HSF2 antibody 10706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Cancer Res

HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma.

Authors - Li-Na Yang
  • KD Validated
View Article