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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSF2BP Polyclonal antibody (32594-1-AP)

    WB analysis using 32594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32594-1-AP (HSF2BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using HSF2BP Polyclonal antibody (32594-1-AP)

    IF Staining of A431 using 32594-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using HSF2BP antibody (32594-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

HSF2BP Polyclonal antibody

HSF2BP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32594-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

MEILB2, Heat shock factor 2-binding protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman testis tissue, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32594-1-AP targets HSF2BP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSF2BP fusion protein Ag37637 相同性解析による交差性が予測される生物種
Full Name heat shock transcription factor 2 binding protein
Calculated molecular weight 38 kDa
Observed molecular weight35 kDa
GenBank accession numberBC000153
Gene Symbol HSF2BP
Gene ID (NCBI) 11077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO75031
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Heat shock factor 2-binding protein (HSF2BP, also known as MEILB2) is a binding protein of HSF2, which is involved in modulating HSF2 activation in testis (PMID: 9651507). Naturally occurring elevated production of HSF2BP in tumors may be a source of cancer-promoting genomic instability and a vulnerable target (PMID: 31960047; 34790765).

Protocols

Product Specific Protocols
WB protocol for HSF2BP antibody 32594-1-APDownload protocol
IF protocol for HSF2BP antibody 32594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols