Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse skeletal muscle tissue, human skeletal muscle tissue, human heart tissue, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
Product Information
18797-1-AP targets HSF4 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock transcription factor 4 |
| Calculated molecular weight | 53 kDa |
| Observed molecular weight | 53-58 kDa |
| GenBank accession number | BC130383 |
| Gene Symbol | HSF4 |
| Gene ID (NCBI) | 3299 |
| RRID | AB_10598014 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULV5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSF4 belongs to the HSF family. HSF4 is a DNA-binding protein that specifically binds heat shock promoter elements (HSE). The isoform HSF4A represses transcription while the isoform HSF4B activates transcription. HSF4 is a homotrimer which exhibits constitutive DNA binding and forms trimers even in the absence of stress. It is expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues. Mutation of HSF4 will cause lamellar cataract which is known as Marner type cataract (CAM). Lamellar cataract is an autosomal dominant common type of infantile cataract. Finger malformation is observed in some kindreds. Inheritance of these forms of cataract is autosomal dominant.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSF4 antibody 18797-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene FGD3 binds with HSF4 to suppress p65 expression and inhibit pancreatic cancer progression.
| ||
Mol Cell Biochem HSF4 promotes tumor progression of colorectal cancer by transactivating c-MET
| ||
Invest Ophthalmol Vis Sci HSF4 Transcriptionally Activates Autophagy by Regulating ATG9a During Lens Terminal Differentiation |