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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSF5 Polyclonal antibody

HSF5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17765-1-AP

Synonyms

HSTF5, HSTF 5, HSF 5

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using HSF5 Polyclonal antibody (17765-1-AP)

    WB analysis of Jurkat using 17765-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17765-1-AP (HSF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HSF5 Polyclonal antibody (17765-1-AP)

    WB analysis of Jurkat using 17765-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17765-1-AP (HSF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17765-1-AP targets HSF5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSF5 fusion protein Ag12168 相同性解析による交差性が予測される生物種
Full Name heat shock transcription factor family member 5
Calculated molecular weight 596 aa, 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC033017
Gene symbol HSF5
Gene ID (NCBI) 124535
RRIDAB_2233301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Heat shock transcription factor 5 (HSF5) is a member of the HSF family, which are well-known transcriptional regulators of genes encoding heat shock proteins and other types of proteins and have functions in reproduction, the immune response, and the aging process. HSF5 is a non-canonical HSF that possesses a winged- helix-turn-helix (WHTH)-like DNA-binding domain and is specifically expressed in the testis (PMID: 38958533, PMID: 39162221).

Protocols

Product Specific Protocols
WB protocol for HSF5 antibody 17765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols