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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSPB2 Polyclonal antibody

HSPB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21755-1-AP

Synonyms

Heat shock protein family B member 2, Heat shock protein beta-2, Heat shock protein beta 2, heat shock 27kDa protein 2, DMPK-binding protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human skeletal muscle tissue using HSPB2 Polyclonal antibody (21755-1-AP)

    WB analysis of human skeletal muscle using 21755-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21755-1-AP (HSPB2 antibody) at dilution of 1:500 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of rat heart tissue using HSPB2 Polyclonal antibody (21755-1-AP)

    WB analysis of rat heart using 21755-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 21755-1-AP (HSPB2 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using HSPB2 Polyclonal antibody (21755-1-AP)

    WB analysis of human skeletal muscle using 21755-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21755-1-AP (HSPB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using HSPB2 Polyclonal antibody (21755-1-AP)

    IHC staining of human heart using 21755-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21755-1-AP (HSPB2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using HSPB2 Polyclonal antibody (21755-1-AP)

    IHC staining of human heart using 21755-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21755-1-AP (HSPB2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using HSPB2 Polyclonal antibody (21755-1-AP)

    IHC staining of human skeletal muscle using 21755-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21755-1-AP (HSPB2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using HSPB2 Polyclonal antibody (21755-1-AP)

    IHC staining of human skeletal muscle using 21755-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21755-1-AP (HSPB2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HSPB2 Polyclonal antibody (21755-1-AP)

    IF Staining of HepG2 using 21755-1-AP

    Immunofluorescent analysis of HepG2 cells, using HSPB2 antibody 21755-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inhuman skeletal muscle tissue, rat heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21755-1-AP targets HSPB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSPB2 fusion protein Ag16146 相同性解析による交差性が予測される生物種
Full Name heat shock 27kDa protein 2
Calculated molecular weight 182 aa, 20 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC109393
Gene symbol HSPB2
Gene ID (NCBI) 3316
RRIDAB_10859789
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HSPB2 antibody 21755-1-APDownload protocol
IHC protocol for HSPB2 antibody 21755-1-APDownload protocol
IF protocol for HSPB2 antibody 21755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cancer

miR-17-5p promotes the invasion and migration of colorectal cancer by regulating HSPB2.

Authors - Weifang Yu
View Article
humanWB,IHC

Clin Epigenetics

PMID: 31088577

Authors - An-An Yin
View Article