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  • Featured Product
  • KD/KO Validated

HSP60 Monoclonal antibody, PBS Only (Capture)

HSP60 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F10E7

Cat no : 66041-1-PBS

Synonyms

HSPD1, GROEL, EC:5.6.1.7, CPN60, Chaperonin 60

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP60 Monoclonal antibody (66041-1-Ig)

    WB analysis using 66041-1-Ig (same clone as 66041-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66041-1-Ig (HSP60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using HSP60 Monoclonal antibody (66041-1-Ig)

    IP experiment of mouse liver using 66041-1-Ig (same clone as 66041-1-PBS)

    IP result of anti-HSP60 (IP:66041-1-Ig, 5ug; Detection:66041-1-Ig 1:2000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HSP60 Monoclonal antibody (66041-1-Ig)

    IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using HSP60 Monoclonal antibody (66041-1-Ig)

    IHC staining of human ovary tumor using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using HSP60 Monoclonal antibody (66041-1-Ig)

    IHC staining of human skin cancer using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66041-1-Ig (HSP60 antibody at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using HSP60 Monoclonal antibody (66041-1-Ig)

    IHC staining of human spleen using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using HSP60 Monoclonal antibody (66041-1-Ig)

    IHC staining of human tonsillitis using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using HSP60 Monoclonal antibody (66041-1-Ig)

    IHC staining of human testis using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66041-1-Ig(HSP60 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSP60 Monoclonal antibody (66041-1-Ig)

    IF Staining of HeLa using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HSP60 Monoclonal antibody (66041-1-Ig)

    IF Staining of HepG2 using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using HSP60 Monoclonal antibody (66041-1-Ig)

    IF Staining of Neuro-2a using 66041-1-Ig (same clone as 66041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using HSP60 antibody (66041-1-Ig, Clone: 2F10E7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66041-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50644-1

    Cytometric bead array standard curve of MP50644-1

    Cytometric bead array standard curve of MP50644-1, HSPD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66041-1-PBS. Detection antibody: 66041-2-PBS. Standard:Ag7848. Range: 1.563-200 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66041-1-PBS targets HSP60 as part of a matched antibody pair:

MP50644-1: 66041-1-PBS capture and 66041-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSP60 fusion protein Ag7848 相同性解析による交差性が予測される生物種
Full Name heat shock 60kDa protein 1 (chaperonin)
Calculated molecular weight 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC003030
Gene symbol HSP60
Gene ID (NCBI) 3329
RRIDAB_11041709
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)