• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

HSPA4 Polyclonal antibody

HSPA4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 21206-1-AP

Synonyms

HSPH2, hsp70RY, Heat shock protein family H member 2, Heat shock 70-related protein APG-2, Heat shock 70 kDa protein 4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HSPA4 Polyclonal antibody (21206-1-AP)

    WB analysis using 21206-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HSPA4 Polyclonal antibody (21206-1-AP)

    WB analysis of HEK-293 using 21206-1-AP

    WB result of HSPA4 antibody (21206-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA4 transfected HEK-293 cells.

  • Western Blot (WB) analysis of NIH/3T3 cells using HSPA4 Polyclonal antibody (21206-1-AP)

    WB analysis of NIH/3T3 using 21206-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using HSPA4 Polyclonal antibody (21206-1-AP)

    WB analysis of mouse testis using 21206-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21206-1-AP (HSPA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using HSPA4 Polyclonal antibody (21206-1-AP)

    IP experiment of mouse brain using 21206-1-AP

    IP result of anti-HSPA4 (IP:21206-1-AP, 4ug; Detection:21206-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HSPA4 Polyclonal antibody (21206-1-AP)

    IHC staining of human colon cancer using 21206-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HSPA4 Polyclonal antibody (21206-1-AP)

    IHC staining of human colon cancer using 21206-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HSPA4 Polyclonal antibody (21206-1-AP)

    IHC staining of human breast cancer using 21206-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HSPA4 Polyclonal antibody (21206-1-AP)

    IHC staining of human breast cancer using 21206-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using HSPA4 Polyclonal antibody (21206-1-AP)

    IHC staining of human pancreas cancer using 21206-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using HSPA4 Polyclonal antibody (21206-1-AP)

    IHC staining of human pancreas cancer using 21206-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21206-1-AP (HSPA4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HSPA4 Polyclonal antibody (21206-1-AP)

    IF Staining of HepG2 using 21206-1-AP

    Immunofluorescent analysis of HepG2 cells, using HSPA4 antibody 21206-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HSPA4 Polyclonal antibody (21206-1-AP)

    IF Staining of MCF-7 using 21206-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HSPA4 antibody (21206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of C6 cells using HSPA4 Polyclonal antibody (21206-1-AP)

    FC experiment of C6 using 21206-1-AP

    1x10^6 C6 cells were intracellularly stained with 0.4 ug HSPA4 Polyclonal antibody (21206-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inC6 cells, HEK-293 cells, mouse testis tissue, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HepG2 cells
Positive FC (Intra) detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21206-1-AP targets HSPA4 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSPA4 fusion protein Ag15581 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 4
Calculated molecular weight 840 aa, 94 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC110861
Gene symbol HSPA4
Gene ID (NCBI) 3308
RRIDAB_10733641
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPA4 (also known as Apg-2, HSP70RY) is a 110 kDa cytosolic protein, a member of the heat-shock protein 110 (HSP110) subfamily of HSP70 proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA4 is ubiquitously expressed and its expression is not heat inducible. Overexpression of HSPA4 has been reported in some leukemia and solid tumors. This antibody well recognized the endogenous HSPA4 protein in mouse brain/ testis tissues. (PMID: 21487003)

Protocols

Product Specific Protocols
WB protocol for HSPA4 antibody 21206-1-APDownload protocol
IHC protocol for HSPA4 antibody 21206-1-APDownload protocol
IF protocol for HSPA4 antibody 21206-1-APDownload protocol
IP protocol for HSPA4 antibody 21206-1-APDownload protocol
FC protocol for HSPA4 antibody 21206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Biochem Cell Biol

Folate deprivation induces cell cycle arrest at G0/G1 phase and apoptosis in hippocampal neuron cells through down-regulation of IGF-1 signaling pathway.

Authors - Yang Yang
View Article
humanELISA

Eur J Clin Invest

Salivary monitoring related to major surgery.

Authors - Ullmann Yehuda Y
View Article
mouseWB

Int J Med Sci

Exercise combined with trimetazidine improves anti-fatal stress capacity through enhancing autophagy and heat shock protein 70 of myocardium in mice.

Authors - Lingjun Jiang
View Article
humanWB

Mol Cell Proteomics

A new cellular interactome of SARS-CoV-2 nucleocapsid protein and its biological implications

Authors - Yuan-Qin Min
View Article