HSPA1B Polyclonal antibody
HSPA1B Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 25405-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, rat brain tissue, HeLa cells, K-562 cells |
| Positive IP detected in | HEK-293 cells, K-562 cells |
| Positive IHC detected in | human tonsillitis tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IF | See 1 publications below |
Product Information
25405-1-AP targets HSPA1B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HSPA1B fusion protein Ag13700 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock 70kDa protein 1B |
| Calculated molecular weight | 70 kDa |
| Observed molecular weight | 60-70 kDa |
| GenBank accession number | BC057397 |
| Gene symbol | HSP70 |
| Gene ID (NCBI) | 3304 |
| RRID | AB_2880063 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
1. What is Hsp70/HSP1A?
HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.
2. FAQs for Hsp70
a. I cannot detect Hsp70 by western blotting
HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.
a. What loading control can I use for cellular stress experiments with Hsp70?
Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSPA1B antibody 25405-1-AP | Download protocol |
| IHC protocol for HSPA1B antibody 25405-1-AP | Download protocol |
| IP protocol for HSPA1B antibody 25405-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Feedback inhibition of cAMP effector signaling by a chaperone-assisted ubiquitin system. | ||
Hum Mol Genet Loss of Ten-Eleven Translocation 2 induces cardiac hypertrophy and fibrosis through modulating ERK signaling pathway. | ||
Oncotarget Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer. | ||
Front Biosci (Landmark Ed) Exosomal miR-22-3p from Mesenchymal Stem Cells Inhibits the Epithelial-Mesenchymal Transition (EMT) of Melanoma Cells by Regulating LGALS1 | ||
Cancer Gene Ther Loss of exosomal miR-200b-3p from hypoxia cancer-associated fibroblasts promotes tumorigenesis and reduces sensitivity to 5-Flourouracil in colorectal cancer via upregulation of ZEB1 and E2F3 |
