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HSP70 Monoclonal antibody, PBS Only

HSP70 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66183-1-PBS
Clone No.4E7E5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, yeast

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

HSPA1A, HSP72, HSP70.1, Heat shock protein family A member 1A, heat shock 70kDa protein 1A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66183-1-PBS targets HSP70 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, yeast samples.

Tested Reactivity human, mouse, rat, yeast
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSP70 fusion protein Ag19111 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 1A
Calculated molecular weight 70 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC009322
Gene Symbol HSP70
Gene ID (NCBI) 3303
RRIDAB_2881578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP0DMV8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker. This antibody well recognized the endogenous HSP70 protein in multiple cell lines. (21373891)

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