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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP70 Monoclonal antibody (66183-1-Ig)

    WB analysis using 66183-1-Ig (same clone as 66183-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using HSP70 Monoclonal antibody (66183-1-Ig)

    WB analysis of Recombinant protein using 66183-1-Ig (same clone as 66183-1-PBS)

    6*His tagged-recombinant HSP70 (291-641 aa) and HSC70 (346-646 aa) proteins were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 antibody), 66442-1-Ig (HSC70 antibody), and 66005-1-Ig (His antibody), respectively, at dilution of 1:50000, incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using HSP70 Monoclonal antibody (66183-1-Ig)

    WB analysis of HEK-293 using 66183-1-Ig (same clone as 66183-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HSP70 Monoclonal antibody (66183-1-Ig)

    WB analysis of HeLa using 66183-1-Ig (same clone as 66183-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using HSP70 Monoclonal antibody (66183-1-Ig)

    WB analysis of HEK-293 using 66183-1-Ig (same clone as 66183-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of whole yeast using HSP70 Monoclonal antibody (66183-1-Ig)

    WB analysis of whole yeast using 66183-1-Ig (same clone as 66183-1-PBS)

    whole yeast cells were subjected to SDS PAGE followed by western blot with 66183-1-Ig (HSP70 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSP70 Monoclonal antibody (66183-1-Ig)

    IHC staining of human liver cancer using 66183-1-Ig (same clone as 66183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66183-1-Ig (HSP70 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HSP70 Monoclonal antibody (66183-1-Ig)

    IHC staining of human breast cancer using 66183-1-Ig (same clone as 66183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66183-1-Ig (HSP70 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HSP70 Monoclonal antibody (66183-1-Ig)

    IHC staining of human breast cancer using 66183-1-Ig (same clone as 66183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66183-1-Ig (HSP70 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using HSP70 Monoclonal antibody (66183-1-Ig)

    IHC staining of human pancreas cancer using 66183-1-Ig (same clone as 66183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66183-1-Ig (HSP70 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSP70 Monoclonal antibody (66183-1-Ig)

    IF Staining of HeLa using 66183-1-Ig (same clone as 66183-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HSP70 antibody (66183-1-Ig, Clone: 4E7E5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using HSP70 Monoclonal antibody (66183-1-Ig)

    FC experiment of HeLa using 66183-1-Ig (same clone as 66183-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human HSP70 (66183-1-Ig, Clone:4E7E5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66183-1-PBS in a different storage buffer formulation.

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HSP70 Monoclonal antibody, PBS Only

HSP70 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66183-1-PBS
Clone No.4E7E5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, yeast

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

HSPA1A, 4E7E5, Heat shock 70 kDa protein 1A, heat shock 70kDa protein 1A, Heat shock protein family A member 1A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66183-1-PBS targets HSP70 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, yeast samples.

Tested Reactivity human, mouse, rat, yeast
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSP70 fusion protein Ag19111 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 1A
Calculated molecular weight 70 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC009322
Gene Symbol HSP70
Gene ID (NCBI) 3303
RRIDAB_2881578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP0DMV8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker. This antibody well recognized the endogenous HSP70 protein in multiple cell lines. (21373891)