HSP70 Recombinant antibody
HSP70 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat, yeast
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
4E10
Cat no : 80735-1-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells, NIH/3T3 cells, C6 cells, the whole yeast |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80735-1-RR targets HSP70 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, yeast samples.
| Tested Reactivity | Human, mouse, rat, yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | HSP70 fusion protein Ag1446 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock 70kDa protein 1A |
| Calculated molecular weight | 70 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC009322 |
| Gene symbol | HSP70 |
| Gene ID (NCBI) | 3303 |
| RRID | AB_2918908 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSP70 antibody 80735-1-RR | Download protocol |
| IHC protocol for HSP70 antibody 80735-1-RR | Download protocol |
| IF protocol for HSP70 antibody 80735-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
