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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSP70 Recombinant antibody

HSP70 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, yeast

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4E10

Cat no : 80735-1-RR

Synonyms

HSPA1A, HSP72, HSP70.1, Heat shock protein family A member 1A, heat shock 70kDa protein 1A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP70 Recombinant antibody (80735-1-RR)

    WB analysis using 80735-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80735-1-RR (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HSP70 Recombinant antibody (80735-1-RR)

    IHC staining of human breast cancer using 80735-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80735-1-RR (HSP70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HSP70 Recombinant antibody (80735-1-RR)

    IHC staining of human liver cancer using 80735-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80735-1-RR (HSP70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HSP70 Recombinant antibody (80735-1-RR)

    IHC staining of human colon cancer using 80735-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80735-1-RR (HSP70 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSP70 Recombinant antibody (80735-1-RR)

    IF Staining of HeLa using 80735-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP70 antibody (80735-1-RR, Clone: 4E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, NIH/3T3 cells, C6 cells, the whole yeast
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80735-1-RR targets HSP70 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, yeast samples.

Tested Reactivity human, mouse, rat, yeast
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HSP70 fusion protein Ag1446 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 1A
Calculated molecular weight 70 kDa
Observed molecular weight70 kDa
GenBank accession numberBC009322
Gene symbol HSP70
Gene ID (NCBI) 3303
RRIDAB_2918908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker

Protocols

Product Specific Protocols
WB protocol for HSP70 antibody 80735-1-RRDownload protocol
IHC protocol for HSP70 antibody 80735-1-RRDownload protocol
IF protocol for HSP70 antibody 80735-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols