HSP90 Monoclonal antibody
HSP90 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig and More (2)
Applications
WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA
Conjugate
Unconjugated
CloneNo.
3F11C1
Cat no : 60318-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, ROS1728 cells, NIH/3T3 cells, 4T1 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 83 publications below |
| IHC | See 4 publications below |
| IF | See 10 publications below |
| IP | See 5 publications below |
| CoIP | See 4 publications below |
| RIP | See 1 publications below |
Product Information
60318-1-Ig targets HSP90 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HSP90 fusion protein Ag3826 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock protein 90kDa alpha (cytosolic), class A member 1 |
| Calculated molecular weight | 853 aa, 90 kDa |
| Observed molecular weight | 85-90 kDa |
| GenBank accession number | BC023006 |
| Gene symbol | HSP90 |
| Gene ID (NCBI) | 3320 |
| RRID | AB_2881429 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSP90 antibody 60318-1-Ig | Download protocol |
| IHC protocol for HSP90 antibody 60318-1-Ig | Download protocol |
| IF protocol for HSP90 antibody 60318-1-Ig | Download protocol |
| IP protocol for HSP90 antibody 60318-1-Ig | Download protocol |
| FC protocol for HSP90 antibody 60318-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia | ||
Aging Cell Urolithin A improves mitochondrial health, reduces cartilage degeneration, and alleviates pain in osteoarthritis. | ||
Mol Cancer Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation. | ||
Redox Biol Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma. | ||
Cell Death Dis HOXC11 drives lung adenocarcinoma progression through transcriptional regulation of SPHK1 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Xiao (Verified Customer) (06-03-2024) | Works well in many cell types
|
FH Boyan (Verified Customer) (10-05-2021) | This antibody recognized a strong band around 90 kd, as shown in the website.
|
FH Ayyappan (Verified Customer) (01-16-2019) | This antibody works well for IP in human cancer cell lines
|
