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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSP90 Recombinant antibody, PBS Only

HSP90 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241050A10

Cat no : 83950-5-PBS

Synonyms

HSP90AA1, HSP90A, Heat shock protein HSP 90-alpha, Heat shock protein HSP 90 alpha, Heat shock protein family C member 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP90 Recombinant antibody (83950-5-RR)

    WB analysis using 83950-5-RR (same clone as 83950-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83950-5-RR (HSP90 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83950-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HSP90 Recombinant antibody (83950-5-RR)

    IF Staining of HepG2 using 83950-5-RR (same clone as 83950-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90 antibody (83950-5-RR, Clone: 241050A10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83950-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using HSP90 Recombinant antibody (83950-5-RR)

    FC experiment of Jurkat using 83950-5-RR (same clone as 83950-5-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug HSP90 Recombinant antibody (83950-5-RR, Clone:241050A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83950-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83950-5-RR

    Affinity and Kinetic Characterization of 83950-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83950-5-RR against Human HSP90 were performed. The affinity constant is 0.692 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83950-5-PBS is HSP90 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HSP90 fusion protein Ag3826 相同性解析による交差性が予測される生物種
Full Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Calculated molecular weight 853 aa, 90 kDa
GenBank accession numberBC023006
Gene symbol HSP90
Gene ID (NCBI) 3320
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.