Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 11405-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 11405-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11405-1-AP
WB result of HSP90AB1 antibody (11405-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells.
WB analysis using 11405-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of NIH/3T3 using 11405-1-AP
IP result of anti-HSP90AB1 (IP:11405-1-AP, 4ug; Detection:11405-1-AP 1:700) with NIH/3T3 cells lysate 1200ug.
IHC staining of human pancreas cancer using 11405-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 11405-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 11405-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11405-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
IHC staining of human breast cancer using 11405-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
IF Staining of HepG2 using 11405-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90AB1 antibody (11405-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 11405-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug HSP90AB1 Polyclonal antibody (11405-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

HSP90AB1 Polyclonal antibody

HSP90AB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11405-1-AP

Publications(35)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Synonyms

HSPC3, HSPC2, HSP90B, Heat shock protein HSP 90-beta, Heat shock protein HSP 90 beta

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (35)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	PC-3 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, MCF-7 cells, HeLa cells, mouse thymus tissue, rat thymus tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IP detected in	NIH/3T3 cells
Positive IHC detected in	human pancreas cancer tissue, human breast cancer tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 32 publications below
IHC	See 4 publications below
IF	See 2 publications below
IP	See 4 publications below
CoIP	See 2 publications below
RIP	See 1 publications below

Product Information

11405-1-AP targets HSP90AB1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken, sheep, deer
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HSP90AB1 fusion protein Ag1947 相同性解析による交差性が予測される生物種
Full Name	heat shock protein 90kDa alpha (cytosolic), class B member 1
Calculated molecular weight	723 aa, 83 kDa
Observed molecular weight	83-90 kDa
GenBank accession number	BC014485
Gene Symbol	HSP90AB1
Gene ID (NCBI)	3326
RRID	AB_2121207
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P08238
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

Protocols

Product Specific Protocols
WB protocol for HSP90AB1 antibody 11405-1-AP	Download protocol
IHC protocol for HSP90AB1 antibody 11405-1-AP	Download protocol
IF protocol for HSP90AB1 antibody 11405-1-AP	Download protocol
IP protocol for HSP90AB1 antibody 11405-1-AP	Download protocol
FC protocol for HSP90AB1 antibody 11405-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Cell Res Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. Authors - Shaojun Zhang View Article
pig	WB	Autophagy Induction of autophagy and suppression of type I IFN secretion by CSFV. Authors - Baoming Xie View Article
	WB	Cell Rep CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons Authors - Carissa L Sirois View Article
human	WB	Haematologica Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines. Authors - Stephan Michalik View Article
human	WB	Anal Chem Solvent-Induced Protein Precipitation for Drug Target Discovery on the Proteomic Scale. Authors - Xiaolei Zhang View Article
sheep	IF	PLoS Pathog A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion. Authors - Thomas Labadie View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11405-1-AP

WB analysis using 11405-1-AP

WB analysis of HEK-293 using 11405-1-AP

WB analysis using 11405-1-AP

IP experiment of NIH/3T3 using 11405-1-AP

IHC staining of human pancreas cancer using 11405-1-AP

IHC staining of human pancreas cancer using 11405-1-AP

IHC staining of human colon using 11405-1-AP

IHC staining of human breast cancer using 11405-1-AP

IHC staining of human breast cancer using 11405-1-AP

IF Staining of HepG2 using 11405-1-AP

FC experiment of HeLa using 11405-1-AP

HSP90AB1 Polyclonal antibody

HSP90AB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Induction of autophagy and suppression of type I IFN secretion by CSFV.

CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Solvent-Induced Protein Precipitation for Drug Target Discovery on the Proteomic Scale.

A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion.