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GRP94 Polyclonal antibody

GRP94 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10979-1-AP

Synonyms

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP


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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue, human appendicitis tissue
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10979-1-AP targets GRP94 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRP94 fusion protein Ag1439 相同性解析による交差性が予測される生物種
Full Name heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight 96 kDa
Observed molecular weight 94 kDa
GenBank accession numberBC009195
Gene symbol GRP94
Gene ID (NCBI) 7184
RRIDAB_2119057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).

Protocols

Product Specific Protocols
WB protocol for GRP94 antibody 10979-1-APDownload protocol
IHC protocol for GRP94 antibody 10979-1-APDownload protocol
IF protocol for GRP94 antibody 10979-1-APDownload protocol
IP protocol for GRP94 antibody 10979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neuroinflammation

TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease.

Authors - Na Liu
human,pigWB

Vet Microbiol

ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells.

Authors - Liang Zhang
ratWB

Mol Med Rep

Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.

Authors - Maojun Liu
mouseIHC,IF,WB

J Immunol Res

Negative Feedback of the cAMP/PKA Pathway Regulates the Effects of Endoplasmic Reticulum Stress-Induced NLRP3 Inflammasome Activation on Type II Alveolar Epithelial Cell Pyroptosis as a Novel Mechanism of BLM-Induced Pulmonary Fibrosis

Authors - Qiaohui Hong
humanWB

Carcinogenesis

Extracellular Vesicles Long RNA Profiling Identifies Abundant mRNA, circRNA and lncRNA in Human Bile as Potential Biomarkers for Cancer Diagnosis

Authors - Huajie Zong
humanWB

Exp Brain Res

CircNCOA4 knockdown attenuates OGD-induced neuron injury through miR-338-5p/PDE4B axis

Authors - Danyang Wang