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  • Featured Product
  • KD/KO Validated

GRP94 Polyclonal antibody

GRP94 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10979-1-AP

Synonyms

Cell and organelle markers, ECGP, endoplasmic reticulum marker, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

Validation Data Gallery

Applications:
  • WB analysis using 10979-1-AP

    WB analysis using 10979-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10979-1-AP (GRP94 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 10979-1-AP

    WB analysis of HEK-293 using 10979-1-AP

    WB result of GPR94 antibody (10979-1-AP, 1:1000) with si-control and si-GPR94 transfected HEK293 cells..

  • WB analysis of MCF-7 using 10979-1-AP

    WB analysis of MCF-7 using 10979-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 10979-1-AP

    WB analysis of A549 using 10979-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 10979-1-AP

    WB analysis of K-562 using 10979-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 10979-1-AP

    WB analysis of NIH/3T3 using 10979-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10979-1-AP (HSP90B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 10979-1-AP

    IP experiment of MCF-7 using 10979-1-AP

    IP result of anti-GRP94 (IP:10979-1-AP, 4ug; Detection:10979-1-AP 1:500) with MCF-7 cells lysate 4000ug.

  • IHC staining of human breast cancer using 10979-1-AP

    IHC staining of human breast cancer using 10979-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10979-1-AP (HSP90B1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 10979-1-AP

    IHC staining of human breast cancer using 10979-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10979-1-AP (HSP90B1 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human lung cancer using 10979-1-AP

    IHC staining of human lung cancer using 10979-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 10979-1-AP (HSP90B1 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of MCF-7 using 10979-1-AP

    IF Staining of MCF-7 using 10979-1-AP

    Immunofluorescent analysis of MCF-7 cells using 10979-1-AP (GRP94 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human appendicitis using 10979-1-AP

    IF Staining of human appendicitis using 10979-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse pancreas using 10979-1-AP

    IF Staining of mouse pancreas using 10979-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using GRP94 antibody (10979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HEK-293 using 10979-1-AP

    IF Staining of HEK-293 using 10979-1-AP

    Immunofluorescent analysis of HEK-293 cells using 10979-1-AP (GRP94 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of MCF-7 using 10979-1-AP

    FC experiment of MCF-7 using 10979-1-AP

    1X10^6 MCF-7 cells were stained with 0.2ug GRP94 antibody (10979-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, K-562 cells, NIH/3T3 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue, MCF-7 cells, HEK-293 cells, human appendicitis tissue
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

10979-1-AP targets GRP94 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRP94 fusion protein Ag1439 相同性解析による交差性が予測される生物種
Full Name heat shock protein 90kDa beta (Grp94), member 1
Calculated molecular weight 96 kDa
Observed molecular weight 94 kDa
GenBank accession numberBC009195
Gene symbol HSP90B1
Gene ID (NCBI) 7184
RRIDAB_2119057
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.

Protocols

Product Specific Protocols
WB protocol for GRP94 antibody 10979-1-APDownload protocol
IHC protocol for GRP94 antibody 10979-1-APDownload protocol
IF protocol for GRP94 antibody 10979-1-APDownload protocol
IP protocol for GRP94 antibody 10979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neuroinflammation

TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease.

Authors - Na Liu
View Article
human,pigWB

Vet Microbiol

ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells.

Authors - Liang Zhang
View Article
ratWB

Mol Med Rep

Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.

Authors - Maojun Liu
View Article
mouseIHC,IF,WB

J Immunol Res

Negative Feedback of the cAMP/PKA Pathway Regulates the Effects of Endoplasmic Reticulum Stress-Induced NLRP3 Inflammasome Activation on Type II Alveolar Epithelial Cell Pyroptosis as a Novel Mechanism of BLM-Induced Pulmonary Fibrosis

Authors - Qiaohui Hong
View Article
humanWB

Carcinogenesis

Extracellular Vesicles Long RNA Profiling Identifies Abundant mRNA, circRNA and lncRNA in Human Bile as Potential Biomarkers for Cancer Diagnosis

Authors - Huajie Zong
View Article
humanWB

Exp Brain Res

CircNCOA4 knockdown attenuates OGD-induced neuron injury through miR-338-5p/PDE4B axis

Authors - Danyang Wang
View Article