Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
12667-2-AP targets HSPA13 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HSPA13 fusion protein Ag3349 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock protein 70kDa family, member 13 |
| Calculated molecular weight | 471 aa, 52 kDa |
| Observed molecular weight | 60, 70 kDa |
| GenBank accession number | BC036370 |
| Gene Symbol | HSPA13 |
| Gene ID (NCBI) | 6782 |
| RRID | AB_2119204 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48723 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSPA13 (Heat shock 70 kDa protein 13), also known as STCH (the stress 70 protein chaperone), is a member of the Hsp70 family of ATPase "stress" or "heat shock" proteins. Human STCH encodes a 60 kDa protein that is highly enriched in the ER. Recently it has been reported that overexpression of the HSPA13 gene reduces prion disease incubation time in mice.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSPA13 antibody 12667-2-AP | Download protocol |
| IHC protocol for HSPA13 antibody 12667-2-AP | Download protocol |
| IP protocol for HSPA13 antibody 12667-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv HSPA13 facilitates NF-κB-mediated transcription and attenuates cell death responses in TNFα signaling.
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Proc Natl Acad Sci U S A Overexpression of the Hspa13 (Stch) gene reduces prion disease incubation time in mice. | ||
Endocrinology Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma. | ||
Proteome Sci PRM-based quantitative proteomics analysis of altered HSP abundance in villi and decidua of patients with early missed abortion | ||
Cell Death Discov Heat shock protein HSPA13 promotes hepatocellular carcinoma progression by stabilizing TANK
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