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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSPA13 Recombinant antibody (84426-5-RR)

    WB analysis using 84426-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84426-5-RR (HSPA13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84426-5-RR

    Affinity and Kinetic Characterization of 84426-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84426-5-RR against Human HSPA13 were performed. The affinity constant is 39.3 nM.

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HSPA13 Recombinant antibody

HSPA13 Recombinant Antibody for WB, ELISA
Cat No. 84426-5-RR
Clone No.241572E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Stress-70 protein chaperone microsome-associated 60 kDa protein, STCH, Microsomal stress-70 protein ATPase core, Heat shock protein family A member 13, Heat shock 70 kDa protein 13

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, fetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84426-5-RR targets HSPA13 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HSPA13 fusion protein Ag3349 相同性解析による交差性が予測される生物種
Full Name heat shock protein 70kDa family, member 13
Calculated molecular weight 471 aa, 52 kDa
Observed molecular weight60 kDa
GenBank accession numberBC036370
Gene Symbol HSPA13
Gene ID (NCBI) 6782
RRIDAB_3671953
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP48723
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPA13 (Heat shock 70 kDa protein 13), also known as STCH (the stress 70 protein chaperone), is a member of the Hsp70 family of ATPase "stress" or "heat shock" proteins. Human STCH encodes a 60 kDa protein that is highly enriched in the ER. Recently it has been reported that overexpression of the HSPA13 gene reduces prion disease incubation time in mice.

Protocols

Product Specific Protocols
WB protocol for HSPA13 antibody 84426-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols