• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSPA14 Polyclonal antibody

HSPA14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28503-1-AP

Synonyms

HSP70 4, HSP60, Heat shock protein HSP60, Heat shock protein family A member 14, heat shock 70kDa protein 14

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HSPA14 Polyclonal antibody (28503-1-AP)

    WB analysis using 28503-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28503-1-AP (HSPA14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using HSPA14 Polyclonal antibody (28503-1-AP)

    IHC staining of human ovary cancer using 28503-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28503-1-AP (HSPA14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using HSPA14 Polyclonal antibody (28503-1-AP)

    IF Staining of K-562 using 28503-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HSPA14 antibody (28503-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28503-1-AP targets HSPA14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSPA14 fusion protein Ag27240 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 14
Observed molecular weight 55 kDa
GenBank accession numberBC119690
Gene symbol HSPA14
Gene ID (NCBI) 51182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HSPA14 antibody 28503-1-APDownload protocol
IHC protocol for HSPA14 antibody 28503-1-APDownload protocol
IF protocol for HSPA14 antibody 28503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols