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HSPA1L Polyclonal antibody
HSPA1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 13970-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue, MCF-7 cells, HeLa cells, HepG2 cells, human saliva tissue, rat testis tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human endometrial cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IF | See 2 publications below |
Product Information
13970-1-AP targets HSPA1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HSPA1L fusion protein Ag4995 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock 70kDa protein 1-like |
| Calculated molecular weight | 70 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC034483 |
| Gene symbol | HSPA1L |
| Gene ID (NCBI) | 3305 |
| RRID | AB_2119675 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognized the endogenous HSPA1L protein in multiple cell lines. (21730052)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSPA1L antibody 13970-1-AP | Download protocol |
| IHC protocol for HSPA1L antibody 13970-1-AP | Download protocol |
| IF protocol for HSPA1L antibody 13970-1-AP | Download protocol |
| IP protocol for HSPA1L antibody 13970-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Endocrinol (Lausanne) Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring. | ||
Virol J Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9. | ||
PeerJ The heat shock protein family gene Hspa1l in male mice is dispensable for fertility.
| ||
Neurobiol Dis SBT-272 improves TDP-43 pathology in ALS upper motor neurons by modulating mitochondrial integrity, motility, and function | ||
J Cachexia Sarcopenia Muscle The regulation of MFG-E8 on the mitophagy in diabetic sarcopenia via the HSPA1L-Parkin pathway and the effect of D-pinitol | ||
Biomolecules Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model |
