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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSPA1L Monoclonal antibody, PBS Only

HSPA1L Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E7H2

Cat no : 66780-1-PBS

Synonyms

Heat shock 70 kDa protein 1L, HSP70 1L, HSP70 HOM, HSP70T, HSPA1L, hum70t

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HSPA1L Monoclonal antibody (66780-1-Ig)

    WB analysis of HeLa using 66780-1-Ig (same clone as 66780-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HSPA1L Monoclonal antibody (66780-1-Ig)

    WB analysis of HepG2 using 66780-1-Ig (same clone as 66780-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using HSPA1L Monoclonal antibody (66780-1-Ig)

    WB analysis of L02 using 66780-1-Ig (same clone as 66780-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using HSPA1L Monoclonal antibody (66780-1-Ig)

    WB analysis of Jurkat using 66780-1-Ig (same clone as 66780-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using HSPA1L Monoclonal antibody (66780-1-Ig)

    WB analysis of HSC-T6 using 66780-1-Ig (same clone as 66780-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using HSPA1L Monoclonal antibody (66780-1-Ig)

    WB analysis of NIH/3T3 using 66780-1-Ig (same clone as 66780-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66780-1-Ig (HSPA1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSPA1L Monoclonal antibody (66780-1-Ig)

    IHC staining of mouse testis using 66780-1-Ig (same clone as 66780-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66780-1-Ig (HSPA1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using HSPA1L Monoclonal antibody (66780-1-Ig)

    IHC staining of mouse testis using 66780-1-Ig (same clone as 66780-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66780-1-Ig (HSPA1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HSPA1L Monoclonal antibody (66780-1-Ig)

    IF Staining of HeLa using 66780-1-Ig (same clone as 66780-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66780-1-Ig (HSPA1L antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66780-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66780-1-PBS targets HSPA1L in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HSPA1L fusion protein Ag5182 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 1-like
Calculated molecular weight 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC034483
Gene symbol HSPA1L
Gene ID (NCBI) 3305
RRIDAB_2882125
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HSPA1L (Hsp70-like protein 1, also known as HSP70-hom) is a member of Hsp70 family of heat shock proteins. Recently it has been reported that HSPA1L can interact with TLR4, activate dendritic cells and induce Th1 Response. This antibody well recognizes the endogenous HSPA1L protein in multiple cell lines. (21730052)