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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSPA2 Monoclonal antibody, PBS Only

HSPA2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A4A9

Cat no : 66291-1-PBS

Synonyms

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HSPA2 Monoclonal antibody (66291-1-Ig)

    WB analysis using 66291-1-Ig (same clone as 66291-1-PBS)

    Various lysates (25 μg/lane) were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using HSPA2 Monoclonal antibody (66291-1-Ig)

    WB analysis of ROS1728 using 66291-1-Ig (same clone as 66291-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HSPA2 Monoclonal antibody (66291-1-Ig)

    WB analysis of HeLa using 66291-1-Ig (same clone as 66291-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66291-1-Ig (HSPA2 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HSPA2 Monoclonal antibody (66291-1-Ig)

    IHC staining of human prostate cancer using 66291-1-Ig (same clone as 66291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66291-1-Ig (HSPA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using HSPA2 Monoclonal antibody (66291-1-Ig)

    IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using HSPA2 Monoclonal antibody (66291-1-Ig)

    IHC staining of human testis using 66291-1-Ig (same clone as 66291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66291-1-Ig (HSPA2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HSPA2 Monoclonal antibody (66291-1-Ig)

    IHC staining of human prostate cancer using 66291-1-Ig (same clone as 66291-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66291-1-Ig (HSPA2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66291-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66291-1-PBS targets HSPA2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSPA2 fusion protein Ag24228 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 2
Calculated molecular weight 639 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC036107
Gene symbol HSPA2
Gene ID (NCBI) 3306
RRIDAB_2881674
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)