Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, A549 cells, HaCaT cells, NIH/3T3 cells, human kidney tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83466-1-RR targets HSPA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | HSPA2 fusion protein Ag3539 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock 70kDa protein 2 |
| Calculated molecular weight | 639 aa, 70 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC036107 |
| Gene Symbol | HSPA2 |
| Gene ID (NCBI) | 3306 |
| RRID | AB_3671096 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P54652 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. At physiological temperature, the HSPA2 was localized primarily in cytoplasm whereas, during heat shock, localization shifted to nucleus and nucleoli (PMID: 18452162). HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HSPA2 antibody 83466-1-RR | Download protocol |
| IHC protocol for HSPA2 antibody 83466-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |