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  • KD/KO Validated

HSPA2 Recombinant antibody

HSPA2 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240421A2

Cat no : 83466-1-RR

Synonyms

HSP70 2, Heat shock-related 70 kDa protein 2, Heat shock protein family A member 2, Heat shock 70 kDa protein 2, 240421A2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HSPA2 Recombinant antibody (83466-1-RR)

    WB analysis of HeLa using 83466-1-RR

    WB result of HSPA2 antibody (83466-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA2 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using HSPA2 Recombinant antibody (83466-1-RR)

    WB analysis using 83466-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83466-1-RR (HSPA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83466-1-RR

    Affinity and Kinetic Characterization of 83466-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83466-1-RR against Human HSPA2 were performed. The affinity constant is 1.86 nM.

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Tested Applications

Positive WB detected inHeLa cells, A431 cells, A549 cells, HaCaT cells, NIH/3T3 cells, human kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83466-1-RR is HSPA2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HSPA2 fusion protein Ag3539 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 2
Calculated molecular weight 639 aa, 70 kDa
Observed molecular weight70 kDa
GenBank accession numberBC036107
Gene symbol HSPA2
Gene ID (NCBI) 3306
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. At physiological temperature, the HSPA2 was localized primarily in cytoplasm whereas, during heat shock, localization shifted to nucleus and nucleoli (PMID: 18452162). HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)

Protocols

Product Specific Protocols
WB protocol for HSPA2 antibody 83466-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols