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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSPA4L Polyclonal antibody (32468-1-AP)

    WB analysis using 32468-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32468-1-AP (HSPA4L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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HSPA4L Polyclonal antibody

HSPA4L Polyclonal Antibody for WB, ELISA
Cat No. 32468-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHL-60 cells, K-562 cells, RT-4 cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32468-1-AP targets HSPA4L in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSPA4L fusion protein Ag38354 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 4-like
Calculated molecular weight95kDa，839aa
Observed molecular weight95~100 kDa
GenBank accession numberNM_001317381.1
Gene Symbol HSPA4L
Gene ID (NCBI) 22824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO95757
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPA4L (Heat shock 70-kDa protein 4-like), also known as APG1 or OSP94, is a member of the heat shock protein 110 family, whose mRNA is strongly expressed in normal human testis and overexpressed in leukemia cells. HSPA4L was also restrictedly expressed in normal tissues and the its product was capable of eliciting a humoral immune response likely induced by its high-level expression in leukemia cells. Up-regulation of the Hspa4l gene in kidney and renal cell lines by osmotic stress suggested that Hspa4l may function to enable the kidney to compensate for osmotic stress present within the kidney. HSPA4L was also restrictedly expressed in normal tissues and the its product was capable of eliciting a humoral immune response likely induced by its high-level expression in leukemia cells (PMID: 16923965, 17588478).

Protocols

Product Specific Protocols
WB protocol for HSPA4L antibody 32468-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols