• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HSPA6 Monoclonal antibody (68257-1-Ig)

    WB analysis using 68257-1-Ig (same clone as 68257-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68257-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HSPA6 Monoclonal antibody (68257-1-Ig)

    WB analysis of HeLa using 68257-1-Ig (same clone as 68257-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68257-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using HSPA6 Monoclonal antibody (68257-1-Ig)

    WB analysis of HEK-293 using 68257-1-Ig (same clone as 68257-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68257-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using HSPA6 Monoclonal antibody (68257-1-Ig)

    IF Staining of A431 using 68257-1-Ig (same clone as 68257-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using HSPA6 antibody (68257-1-Ig, Clone: 3D12D7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68257-1-PBS in a different storage buffer formulation.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

HSPA6 Monoclonal antibody, PBS Only

HSPA6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68257-1-PBS
Clone No.3D12D7

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B, HSP70B, HSPA6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68257-1-PBS targets HSPA6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HSPA6 fusion protein Ag4716 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 6 (HSP70B')
Calculated molecular weight 643 aa, 71 kDa
Observed molecular weight 68-71 kDa
GenBank accession numberBC035665
Gene Symbol HSPA6
Gene ID (NCBI) 3310
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17066
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HSPA6 (also known as HSP70B) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Human HSP70B is known to be strictly inducible and shows very low or no basal expression levels in most cells. HSPA6 induction has been exploited as a reasonable biomarker of cellular stresses and its mRNA expression levels have been shown to be significantly induced against a variety of cellular stresses. This antibody was raised against the C-terminal region of human HSPA6 and it may cross-react with HSPA1 (HSP70), HSPA1L and HSPA2. (18229458)