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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSPB11 Monoclonal antibody (68059-1-Ig)

    WB analysis using 68059-1-Ig (same clone as 68059-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68059-1-Ig (HSPB11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68059-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HSPB11 Monoclonal antibody (68059-1-Ig)

    WB analysis of HeLa using 68059-1-Ig (same clone as 68059-1-PBS)

    WB result of HSPB11 antibody (68059-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPB11 transfected HeLa cells. This data was developed using the same antibody clone with 68059-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

HSPB11 Monoclonal antibody, PBS Only

HSPB11 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68059-1-PBS
Clone No.2D11B3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Heat shock protein beta-11, Heat shock protein beta 11, FAP232, CFAP232, C1orf41

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68059-1-PBS targets HSPB11 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HSPB11 fusion protein Ag8804 相同性解析による交差性が予測される生物種
Full Name heat shock protein family B (small), member 11
Calculated molecular weight 144 aa, 16 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC005245
Gene Symbol HSPB11
Gene ID (NCBI) 51668
RRIDAB_2918800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y547
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.