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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSP20 Polyclonal antibody

HSP20 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 16813-1-AP

Synonyms

Heat shock protein beta 6, Hsp20, HSPB6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP20 Polyclonal antibody (16813-1-AP)

    WB analysis using 16813-1-AP

    rat heart tissues were subjected to SDS PAGE followed by western blot with 16813-1-AP (HSP20 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HSP20 Polyclonal antibody (16813-1-AP)

    WB analysis using 16813-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16813-1-AP (HSP20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using HSP20 Polyclonal antibody (16813-1-AP)

    IHC staining of mouse heart using 16813-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16813-1-AP (HSP20 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using HSP20 Polyclonal antibody (16813-1-AP)

    IHC staining of mouse heart using 16813-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16813-1-AP (HSP20 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HSP20 Polyclonal antibody (16813-1-AP)

    IHC staining of mouse brain using 16813-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16813-1-AP (HSP20 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HSP20 Polyclonal antibody (16813-1-AP)

    IHC staining of mouse brain using 16813-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16813-1-AP (HSP20 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using HSP20 Polyclonal antibody (16813-1-AP)

    IF Staining of mouse heart using 16813-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using HSP20 antibody (16813-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inrat heart tissue, mouse heart tissue
Positive IHC detected inmouse heart tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16813-1-AP targets HSP20 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP20 fusion protein Ag10352 相同性解析による交差性が予測される生物種
Full Name heat shock protein, alpha-crystallin-related, B6
Calculated molecular weight 160 aa, 17 kDa
Observed molecular weight 18-20 kDa
GenBank accession numberBC068046
Gene symbol HSP20
Gene ID (NCBI) 126393
RRIDAB_2878321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hsp20 is a member of the small heat shock superfamily of proteins that function as chaperones to prevent protein misfolding through adenosine triphosphate−independent processes. It is widely expressed but is most abundant in cardiac, skeletal, and smooth muscles. Phosphorylation of HSP20 at Ser16 was elevated in human failing hearts as well as in murine hearts after I/R injury Indicating its cardioprotective function.

Protocols

Product Specific Protocols
WB protocol for HSP20 antibody 16813-1-APDownload protocol
IHC protocol for HSP20 antibody 16813-1-APDownload protocol
IF protocol for HSP20 antibody 16813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols