HSPB8 Polyclonal antibody

Name: HSPB8 Polyclonal antibody
Brand: Proteintech
SKU: 15287-1-AP

HSPB8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15287-1-AP

Synonyms

E2-induced gene 1 protein, E2IG1, DHMN2, CRYAC, CMT2L

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 15287-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15287-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SGC-7901 using 15287-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15287-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human heart using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 15287-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HEK-293 using 15287-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 15287-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Neuro-2a cells, SGC-7901 cells, mouse brain tissue, HEK-293 cells, mouse heart tissue, rat heart tissue
Positive IHC detected in	human heart tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 11 publications below
IHC	See 2 publications below
IF	See 1 publications below
CoIP	See 2 publications below

Product Information

15287-1-AP targets HSPB8 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HSPB8 fusion protein Ag7456 相同性解析による交差性が予測される生物種
Full Name	heat shock 22kDa protein 8
Calculated molecular weight	22 kDa
Observed molecular weight	20-22 kDa
GenBank accession number	BC002673
Gene symbol	HSPB8
Gene ID (NCBI)	26353
RRID	AB_2248640
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for HSPB8 antibody 15287-1-AP	Download protocol
IHC protocol for HSPB8 antibody 15287-1-AP	Download protocol
IF protocol for HSPB8 antibody 15287-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB, IF	Nat Commun Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover. Authors - Thomas G Martin View Article
mouse	IHC	J Neurosci The α crystallin domain of small heat shock protein b8 (Hspb8) acts as survival and differentiation factor in adult hippocampal neurogenesis. Authors - Ramírez-Rodríguez Gerardo G View Article
human	WB	J Am Heart Assoc Reduction of Filamin C Results in Altered Proteostasis, Cardiomyopathy, and Arrhythmias Authors - Joyce C Ohiri View Article
mouse	WB	J Cell Biochem Pharmacological inhibition of BAG3-HSP70 with the proposed cancer therapeutic JG-98 is toxic for cardiomyocytes. Authors - Thomas G Martin View Article
mouse,human	CoIP	Genes (Basel) BAG3 Alleviates Atherosclerosis by Inhibiting Endothelial-to-Mesenchymal Transition via Autophagy Activation. Authors - Hongtao Diao View Article
human	WB	Cell Rep Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR. Authors - Dawid Krokowski View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSPB8 Polyclonal antibody

HSPB8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 15287-1-AP

WB analysis of HEK-293 using 15287-1-AP

WB analysis of SGC-7901 using 15287-1-AP

WB analysis of mouse brain using 15287-1-AP

IHC staining of human heart using 15287-1-AP

IHC staining of human heart using 15287-1-AP

IHC staining of human kidney using 15287-1-AP

IHC staining of human kidney using 15287-1-AP

IHC staining of human placenta using 15287-1-AP

IHC staining of human placenta using 15287-1-AP

IHC staining of human testis using 15287-1-AP

IHC staining of human testis using 15287-1-AP

IHC staining of human skin using 15287-1-AP

IHC staining of human skin using 15287-1-AP

IHC staining of human brain using 15287-1-AP

IHC staining of human brain using 15287-1-AP

IHC staining of human spleen using 15287-1-AP

IHC staining of human spleen using 15287-1-AP

IHC staining of human lung using 15287-1-AP

IHC staining of human lung using 15287-1-AP

IHC staining of human ovary using 15287-1-AP

IHC staining of human ovary using 15287-1-AP

IF Staining of HEK-293 using 15287-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover.

The α crystallin domain of small heat shock protein b8 (Hspb8) acts as survival and differentiation factor in adult hippocampal neurogenesis.

Reduction of Filamin C Results in Altered Proteostasis, Cardiomyopathy, and Arrhythmias

Pharmacological inhibition of BAG3-HSP70 with the proposed cancer therapeutic JG-98 is toxic for cardiomyocytes.

BAG3 Alleviates Atherosclerosis by Inhibiting Endothelial-to-Mesenchymal Transition via Autophagy Activation.

Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR.