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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSPBAP1 Polyclonal antibody (27631-1-AP)

    WB analysis using 27631-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27631-1-AP (HSPBAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HSPBAP1 Polyclonal antibody (27631-1-AP)

    IHC staining of mouse kidney using 27631-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27631-1-AP (HSPBAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HSPBAP1 Polyclonal antibody (27631-1-AP)

    IHC staining of human prostate cancer using 27631-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27631-1-AP (HSPBAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HSPBAP1 Polyclonal antibody (27631-1-AP)

    IHC staining of human prostate cancer using 27631-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27631-1-AP (HSPBAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HSPBAP1 Polyclonal antibody (27631-1-AP)

    IHC staining of mouse kidney using 27631-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27631-1-AP (HSPBAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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HSPBAP1 Polyclonal antibody

HSPBAP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27631-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Synonyms

HSPB1 associated protein 1, HSPBAP1, PASS1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 1100 ug/ml
20 μL, 1100 ug/ml
150 μL, 1100 ug/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells
Positive IHC detected inmouse kidney tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27631-1-AP targets HSPBAP1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSPBAP1 fusion protein Ag26428 相同性解析による交差性が予測される生物種
Full Name HSPB (heat shock 27kDa) associated protein 1
Calculated molecular weight 55 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC017763
Gene Symbol HSPBAP1
Gene ID (NCBI) 79663
RRIDAB_2880931
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EW2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPBAP1, also named as Protein associated with small stress protein 1, is a widely expressed 488 amino acid protein. HSPBAP1 may play a role in regulating stress response in the living cell(PMID: 11978969). A chromosomal aberration involving HSPBAP1 has been found in a family with renal carcinoma (PMID:12939738). HSPBAP1 has multiple isoforms with MW 55, 50, 32 and 25 kDa.

Protocols

Product Specific Protocols
WB protocol for HSPBAP1 antibody 27631-1-APDownload protocol
IHC protocol for HSPBAP1 antibody 27631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols